Abstract：In this study, the bacterial community composition in 4geographically different location (Shandong Laiwu, SD; Guangdong Shenzhen, GD; Shanghai Laogang, SH; Chongqing Changshengqiao, CQ) was investigated by Illumina MiSeq sequencing targeting V3~V4region of 16S rDNA gene and the link between the bacterial community composition and environmental parameters was analyzed. Results showed that representatives of Alphaproteobacteria and Betaproteobacteria dominated at all 4cover soils, ranging from 12.67%~25.54% and 14.35%~18.88% of the total abundance, respectively. Cover soil of GD had higher bacterial diversity than the others, which suggested by Shannon index (5.52±0.026 Vs 4.76±0.030 of SD, 4.89±0.037 of SH, and 3.43±0.027 of CQ). Genus Sphingomonas dominated at cover soils of SD、GD and SH, accounting for 7.25%、10.67%、11.30% of the total abundance, respectively. Person correlation suggested that groups of Deltaproteobacteria and Gammaproteobacteria highly correlated to total nitrogen (TN) (r=1.00, P<0.001) and total phosphorus (TP) (r=1.00, P<0.001). Redundancy analysis (RDA) further indicated that TN、TP and organic matter (OM) are the important factors in shaping the bacterial community structure.
