Abstract:In order to explore the effect of a new plasticizer-di-isononyl phthalate (DINP) on mice allergic skin inflammation, we used skin exposure method. Fifty six male Balb/C mice were divided into seven groups, in which four groups and exposed to 0, 1.4, 14 and 140mg/kg DINP, two other groups administrated antioxidant melatonin were set to explore oxidative stress effects, and one group was set as saline control. After exposure, the indexes of ear swelling and the weight difference of bilateral ear were determined, and we observed the pathology change through the ear pathology slice. The biomarkers about oxidative stress were tested too. The results showed that the 14and 140mg/kg DINP worsen skin inflammation significantly (P<0.01), and the oxidative stress indexes also changed obviously (P<0.01). These results suggest that a certain concentration of DINP can significantly increase related symptom of skin inflammation caused by allergen. It certifies that a certain concentration DINP has obvious adjuvant effect on allergic skin inflammation in mice. In addition, along with the concentration of DINP increase, the oxidative stress in the bodies of mice is overactive and it causes organism tissues or cells oxidative damage and contributes to the inflammatory response.