Availability of soil Cu and Cd and microbial community structure as affected by applications of amendments
CUI Hong-biao1,2, FAN Yu-chao1, ZHOU Jing2, SHI Yu2, XU Lei2, GUO Xue-tao1, HU You-biao1, GAO Liang-min1
1. School of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, China;
2. Institute of Soil Science, Chinese Academy Sciences, Nanjing 210008, China
Concentrations of available Cu and Cd, soil microbial community structure were investigated with one-time application of charcoal, lime, and apatite after four years in a heavy metal contaminated soil. Results showed that incorporation of amendments significantly increased soil pH, and decreased the concentrations of exchangeable acidity and aluminum. Lime and apatite were more effective than charcoal on transforming Cu and Cd from active to inactive fractions. The Biolog analysis showed that AWCD (average well color development) of charcoal (0.61), lime (0.76), and apatite (0.70) were 1.33, 1.65 and 1.52 times higher than that in the untreated soil at 192h. Moreover, the Shannon index, McIntosh index of soil microbes were higher in the amended soils compared with the control, and followed the order of lime > apatite > charcoal. The results indicated amendments treated soils all increased the abilities of the microbes to use carbon sources. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis showed that the amounts of dominant bacterial were significantly increased with the application of amendments. The Shannon index of soil microbes were increased 0.22, 0.39 and 0.24 in charcoal, lime and apatite compared with the control, respectively. Correlation analysis indicated that soil acidity and availability of Cu and Cd may be the main factors affecting the community structure of soil microbes in the soils.
崔红标, 范玉超, 周静, 时玉, 徐磊, 郭学涛, 胡友彪, 高良敏. 改良剂对土壤铜镉有效性和微生物群落结构的影响[J]. 中国环境科学, 2016, 36(1): 197-205.
CUI Hong-biao, FAN Yu-chao, ZHOU Jing, SHI Yu, XU Lei, GUO Xue-tao, HU You-biao, GAO Liang-min. Availability of soil Cu and Cd and microbial community structure as affected by applications of amendments. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(1): 197-205.
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