Butyric production and metabolic kinetics of a novel cellulose degrading strain
CHI Xue1, LI Jian-zheng1, AI Bin-ling2
1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;
2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, China
A novel anaerobic strain, which could digest cellulose during butyric acid production, was isolated from an enrichment culture of cow dung, pig manure, corn soil and rotten wood. The strain was a bacillus with a length of 7.1~9.1 μm and a diameter of about 1.2 μm. It belonged to Clostridium butyricum and named C. Butyricum DCB. Strain DCB had a specific growth rate of 0.6536h-1 in cellobiose medium at 35℃. In addition to this, the generation time, degradation rate and butyric acid production rate of Strain DCB were 1.06h, 0.1g/(L·h) and 0.06g/(L·h), respectively. In cellulose medium at 35℃, the highest butyric acid convertion ratio was 0.23g/g.
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CHI Xue, LI Jian-zheng, AI Bin-ling. Butyric production and metabolic kinetics of a novel cellulose degrading strain. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(1): 232-237.
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