Isolation, Identification, and nitrogen removal characteristics of oligotrophic aerobic denitrifiers
ZHOU Shi-lei, HUANG Ting-lin, BAI Shi-yuan, HE Xiu-xiu
Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
In order to isolate the aerobic denitrifiers from oligotrophic reservoir system, and study the taxonomic status and nitrogen removal characteristics for providing evidence to remediate the micro-polluted source water. The oligotrophic aerobic denitrifiers were obtained, through enrichment, domestication, and screening processes, and taxonomic statuses were determined by 16S rRNA, and the nitrogen removal characteristics was detected in the pure culture and source water experiment. As a result, 196 strains were isolated, and 14 strains (ZHF2, ZHF3, ZHF5, ZHF6, ZHF8, ZMF2, ZMF5, ZMF6, N299, G107, 81Y, SF9, SF18, and SXF14) demonstrated the perfect nitrogen removal performances. Based on morphological, physiological, and phylogenetic analysis, ZHF3, ZHF5, ZHF6, ZMF2, G107, 81Y, SF18, and SXF14 were identified as Acinetobacter sp.; ZHF2, and ZHF8 were identified as Novosphingobium sp.; ZMF5 was identified as Aquabacterium sp.; ZMF6 was identified as Sphingomonas sp.; N299 was identified as Zoogloea sp.; SF9 was identified as Delftia sp.. The nitrate removal rates of G107 and 81Y reached 98.88% and 99.44%, in 72h. Meanwhile, the oligotrophic aerobic denitrification bacteria G107 and 81Y demonstrated the obvious nitrogen removal performances in nitrite medium, ammonia medium and source water medium. From all the results, the isolations of oligotrophic aerobic denitrifiers enriched the species of aerobic denitrification bacteria, and the perfect nitrogen removal performances provided a significant parameter to remediate the micro-polluted reservoir water system.
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