Analysis of microbial community structure in different partial nitrification system
HOU Ai-yue, LI Jun, BIAN Wei, WANG Meng, ZHENG Lin-xue, ZHANG Yan-zhuo, ZHAO Xin-yan
Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
In order to study the influences of organic carbon source on the microbial community structure in partial nitrification system, the microbial diversity was investigated in two partial nitrification reactors by the construction of bacterial clone library. The influents of two reactors were artificial wastewater with and without organic carbon source, respectively. The results indicated that Proteobacteria and uncultured bacterium were the dominant bacteria of the two studied systems. The differences existed in the structure of the bacterial community of the two systems. Nonetheless, the dominant bacteria and their proportions were similar to each other in the total clones. In addition, the main denitrifer groups were also similar in the two systems. Due to the different concentrations of organic carbon source, the denitrifer genera and their proportions were distinct. The main denitrifer genera of partial nitrification reactor with inorganic wastewater were Nitrosomonas, Nitrospira and Denitratisoma. The proportion of autotrophic nitrifying bacteria in partial nitrification reactor with inorganic wastewater was higher than that with organic wastewater. But in the reactor with inorganic wastewater, the proportion of autotrophic bacteria was lower than heterotrophic bacteria. The main denitrifer genera of partial nitrification reactor with organic wastewater were some denitrifying bacteria of β-Proteobacteria and Nitrosomonas. Additionally, the proportion of the latter in this reactor was relatively low.
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