Through elemental analysis, ultraviolet-visible spectra and fluorescence index, the composition and structure of the humic acid with and without 15 days biological modification were analyzed, and compared the binding function of three kinds of humic acid on 17β-estradiol (E2). And the binding function between 17β-estradiol (E2) and three different kinds of humic acid that before and after bio-modification were then compared. At last, the microbial degradation influences of E2 mediated by humic acid were studied. The elemental analysis results showed that the (N+O)/C values of humic acid (OLHA, OLFA, OSHA) that before bio-modification and humic acid (BLHA, BLFA, BSHA) that after biological modification were 0.801, 1.214, 0.820 and 0.629, 1.080, 0.797, respectively. The ultraviolet-visible spectrum results showed that the values of SUVA254were 0.146, 0.023, 0.073 and 0.179, 0.036, respectively. And the fluorescence index (FI) were 0.723, 3.385, 2.757 and 0.681, 3.017, 1.702, respectively. The above three kinds of characterization analysis results showed that the polarity of humic acid were consistent, suggesting that the polarity of modified humic acid were weaker than former. Moreover, the binding efficiency of 3 mg/L E2 by 5 mgC/L three humic acid before and after the bio-modification within 30h were 31.37%, 4.96%, 25.86% and 37.78%, 6.03%, 29.92%, respectively, which showed that the binding functions of E2 were increased obviously after humic acid bio-modification treatment. The biodegradation efficiency of 3 mg/L E2 by 5 mgC/L three humic acid before and after the bio-modification within 30h were 46.28%, 15.96%, 38.76% and 51.11%, 17.30%, 44.33%, respectively. Meanwhile, the stronger binding function of the same humic acid concentrations on E2, the greater efficiency of combined humic acid mediated microbial degradation of E2.
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