Synergetic effect of aeration and pile temperature on VS and water content removal and biochemical components degradation during short-term aerobic pretreatment process prior to municipal solid waste (MSW) landfilling were investigated. Simulated organic fraction of MSW (OFMSW) was used as substrate. The results showed that VS rapid degradation was obviously occurred in the initial stage (0~6d), accounting for 68%~85% of total VS degraded in the whole experimental period; simultaneously, approximate 15%~26% water was removed in the forms of leachate and vapor, and extending aeration time could evaporate more water. In the initial stage, the temperature of pile increased to thermophilic condition (50 to 55℃) by self-heating, which was more beneficial for carbohydrates degradation than the mesophilic condition (42 to 47℃) of the controlled pile, while was insignificant to crude protein degradation. Yet, crude fat and lignocellulose have higher degradation rate under mesophilic condition (42 to 47℃). As the pretreatment period extended to 14 days, different synergetic operations combined aeration and pile temperature exhibited minor difference on the removal of VS and moisture. This study showed that a 6 day aerobic process with the pile temperature between 50 to 55℃ was a cost-efficient pretreatment method prior to MSW landfilling.