Correlation of LNA and HNA bacteria based on flow cytometric characteristics
LIU Jie, SONG Yu-hao, MA Rui, WANG Ying-ying
Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
In the present study, bacterial concentration and FCM characteristics of LNA and HNA were analyzed in seven different terrestrial environments. Results showed clear separation between LNA and HNA bacteria in both aquatic and soil environments. The LNA abundance in soil (107~108cells/g) was higher than that of freshwater (105~106cells/mL) while the proportion of LNA in soil (29.80%~33.94%) was lower than that of freshwater (42.25%~65.92%), but not underground water (21.60%). Principal component analysis (PCA) indicated that the flow cytometric characteristics of LNA and HNA had distinct differences between freshwater and soil. Correlation analysis further revealed that both green fluorescence (FL1) and side scatter (SSC) signals between LNA and HNA bacteria had a significant correlation (FL1:R2=0.711, P<0.01; SSC:R2=0.762, P<0.01), i.e. co-variation between LNA and HNA. The variance of SSC was higher than that of FL1 in different ecosystems. The results demonstrated that LNA and HNA bacteria were neither physiologically related nor completely independent communities, but rather have a close co-variation.
刘杰, 宋宇昊, 马芮, 王莹莹. 基于流式细胞特性的LNA和HNA细菌相关性研究[J]. 中国环境科学, 2016, 36(3): 865-874.
LIU Jie, SONG Yu-hao, MA Rui, WANG Ying-ying. Correlation of LNA and HNA bacteria based on flow cytometric characteristics. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(3): 865-874.
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