To explore the effects on mice through administration of formaldehyde (FA) by gavage with or without ovalbumin (OVA), which lead to the inflammation in body, the male Balb/c mice were randomly divided into 7groups: the control group (distilled water group), OVA group, 2mg/(kg·d) FA (formaldehyde) group, 20mg/(kg·d) FA group, 200mg/(kg·d) FA group, 200mg/(kg·d) FA+OVA group and 200mg/(kg·d)FA+OVA+ MT (melatonin) group. Mice were gavaged with distilled water and different concentrations of FA solution respectively for 21 days. The mice in OVA group, 200mg/(kg·d) FA+OVA group and 200mg/(kg·d) FA+OVA+MT group were sensitized with OVA in day 6, 13, 20 by intraperitoneal injection, and the mice in 200mg/(kg·d) FA+OVA+MT group were also challenged with 1mg/mL MT by gavage for 21 days. The contents of ROS, MDA and GSH in liver, kidney and lung tissue homogenate were determined. IL-4and IFN-g concentrations in liver were measured by ELISA kits. The results showed that compared with control group, the livers ROS of mice in 200mg/(kg·d) FA group reached a significant level (P<0.05), and MDA increased significantly (P<0.05); GSH content in kidneys decreased significantly (P<0.05). IL-4 content in livers of mice in 200mg/(kg·d) FA+OVA group significantly increased (P<0.01). In 200mg/(kg·d) FA+OVA group, the administration of 10mg/(kg·d) MT can reduce ROS in the livers significantly (P<0.05). In a word, the gavage of 200mg/(kg·d) FA would induce oxidative damage and inflammation in mice. ROS, MDA in 200mg/(kg·d) FA group reached a significant level (P<0.05), on the contrary, GSH content decreased significantly (P<0.05). In 200mg/(kg·d) FA+OVA group, IL-4 content in livers of mice significantly increased (P<0.01), while IFN-g content had no significant change (P>0.05).
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