Impact of difenoconazole on the antioxidases of zebrafish(Danio rerio)
MU Xi-yan1,2, HUANG Ying2, SHEN Gong-ming2, CHAI Ting-ting1, ZHU Li-zhen1, LI Xu-xing2, LEI Yun-lei2, FENG Geng-fei2, LI Ying-ren2, LI Xue-feng1, WANG Cheng-ju1
1. College of Sciences, China Agricultural University, Beijing 100193, China;
2. Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing 100141, China
To investigate the effect of difenoconazole on zebrafish antioxidases, adult zebrafish were exposed to three concentrations of difenoconazole for 15days. The activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) and glutathione reductase(GR) was measured at 3, 8 and 15 days post exposure(dpe) respectively. Results indicated that the brain CAT activity was increased at 3 and 8 dpe but decreased at 15 dpe. 50μg/L or higher difenoconazole could inhibit brain and hepatic GPx activity of zebrafish, and the inhibition in brain was stronger than liver. In addition, brain GR activity was induced by 500μg/L difenoconazole while hepatic GR activity was reduced under the same exposure. These results implicated that 50μg/L could alter the antioxidant system of zebrafish and its effects on fish living in agricultural water areas should be concerned.
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MU Xi-yan, HUANG Ying, SHEN Gong-ming, CHAI Ting-ting, ZHU Li-zhen, LI Xu-xing, LEI Yun-lei, FENG Geng-fei, LI Ying-ren, LI Xue-feng, WANG Cheng-ju. Impact of difenoconazole on the antioxidases of zebrafish(Danio rerio). CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(4): 1242-1249.
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