An anaerobic sequencing batch reactor (ASBR) was used to investigate the short effects of temperature on nitrogen removal by anaerobic ammonium oxidation and denitrification. The activation energy of the coupled reaction contained anammox and denitrification was lower than the single reactor only contained anammox process. The coupled reaction could balance the negative effects of low temperature. Decrease in temperature had more severe effects on anammox process than denitrification process. The relationship between temperature and the maximum specific nitrogen removal rate could accord with Arrhenius equation. The activation energy of coupled reaction was 49.56kJ/mol which was lower than 66.18kJ/mol calculated by anammox reaction at 25~35℃ and anammox was the dominated reaction in the reactor whose contribution rates to nitrogen removal were 61.29%. The activation energy of coupled reaction was 74.91kJ/mol which was lower than 106.40kJ/mol calculated by anammox reaction at 9~25℃. The contribution rates made by denitrification to nitrogen removal were increased with the decreasing of temperature. Denitrification became the dominated reaction when the temperature dropped to 9℃, and its contribution rate to nitrogen removal was 75.10%. The volume of nitrogen removal rate(NRR) would be influenced by temperature when temperature was lower than 25℃.
Chamchoi N, Nitisoravut S, Schmidt J E. Inactivation of anammox communities under concurrent operation of anaerobic ammonium oxidation (anammox) and denitrification [J]. Bioresource Technology, 2008,99(9):3331-3336.
[2]
Mulder A, Van De Graaf A A, Robertson L, et al. Anaerobic ammonium oxidation discovered in a denitrifying fluidized-bed reactor [J]. FEMS Microbiology Ecology, 1995,16(3):177-183.
[3]
Ni S Q, Lee P H, Fessehaie A, et al. Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor [J]. Bioresource technology, 2010,101(6):1792-1799.
[4]
Kalyuzhnyi S, Gladchenko M, Mulder A, et al. New anaerobic process of nitrogen removal [J]. Water Science & Technology, 2006,54(8):163-170.
[5]
Isaka K, Date Y, Kimuar Y, et al. Nitrogen removal performance using anaerobic ammonium oxidation at low temperatures [J]. FEMS Microbiology Letters, 2008,282(1):32-38.
[6]
Leslie C, Grady C L,等.废水生物处理:第二版,改编和扩充 [M]. 北京:化学工业出版社, 2003.
[7]
Strous M, Kuenen J G, Jetten M S M. Key physiology of anaerobic ammonium oxidation [J]. Applied and Environmental microbiology, 1999,65(7):3248-3250.
[8]
Egli K, Fanger U, Alvarez P J, et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate [J]. Archives of Microbiology, 2001,175(3):198-207.
[9]
Yang Y, Zuo J, Shen P, et al. Influence of temperature, pH value and organic substance on activity of ANAMMOX sludge [J]. Environmental Science, 2006,27(4):691-695.
[10]
Toh S, Webb R, Ashbolt N. Enrichment of autotrophic anaerobic ammonium-oxidizing consortia from various wastewaters [J]. Microb Ecol, 2002,43(1):154-167.
[11]
Kumar M, Lin J G. Co-existence of anammox and denitrification for simultaneous nitrogen and carbon removal—strategies and issues [J]. Journal of Hazardous Materials, 2010,178(1):1-9.
Dosta J, Fern Ndez I, V Zquez-Pad N J R, et al. Short-and long-term effects of temperature on the Anammox process [J]. Journal of Hazardous Materials, 2008,154(1-3):688-693.
[17]
Isaka K, Sumino T, Tsuneda S. High nitrogen removal performance at moderately low temperature utilizing anaerobic ammonium oxidation reactions [J]. Journal of Bioscience and Bioengineering, 2007,103(5):486-490.
Ma B, Peng Y, Zhang S, et al. Performance of anammox UASB reactor treating low strength wastewater under moderate and low temperatures [J]. Bioresource Technology, 2013,129(606-611).
[23]
Canion A, Kostka J, Gihring T, et al. Temperature response of denitrification and anammox reveals the adaptation of microbial communities to in situ temperatures in permeable marine sediments that span 50° in latitude [J]. Biogeosciences, 2014, 11(2):309-320.
[24]
Rysgaard S, Glud R N, Risgaard-Petersen N, et al. Denitrification and anammox activity in Arctic marine sediments [J]. Limnol Oceanogr, 2004,49(5):1493-1502.
Gao F, Zhang H, Yang F, et al. The contrast study of anammox-denitrifying system in two non-woven fixed-bed bioreactors (NFBR) treating different low C/N ratio sewage [J]. Bioresource Technology, 2012,114(54-61).
