An anaerobic sequencing batch reactor (ASBR) was operated to investigate the nitrogen removal properties of ANAMMOX process treating sewage with seawater by gradually increasing seawater proportion. The results showed that ANAMMOX bacteria could remain active and maintain high nitrogen removal efficiency in any proportion of seawater through acclimation. When seawater proportion was not more than 40%, the seawater almost had no effect on the stability and the nitrogen removal efficiency of the reactor. Besides, the activity of ANAMMOX bacteria was enhanced. The maximum specific activity of ANAMMOX bacteria achieved in the reactor with 30% seawater, was 21.3% up compared with reactor containing 10% seawater. When seawater proportion was more than 40%, the nitrogen efficiency of reactor decreased. However, with sufficient time to adapt, the efficiency would recover. During this period, the reaction of the reactor could be divided into three phases: sensitive period, interim stable period and recovery period. When seawater proportion was 100%, the nitrogen removal rate of the reactor could be 0.341kgN/(m3·d), which was 73.7% of that contained 10% seawater, and the nitrogen removal capability had a further recovery trend.
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