An anaerobic sequencing batch reactor (ASBR) was operated to investigate the effects of nitrite on nitrogen removal via ANAMMOX by keeping influent ammonia nitrogen concentration as 110mg/L and increasing influent nitrite concentration at whole seawater condition. The kinetics features were also studied at the same time. ANAMMOX process began to be inhibited when the influent nitrite concentration was 170mg/L, and the ammonia nitrogen removal efficiency decreased 8.41%. The fitting of modified Logistic process kinetics model showed that influent nitrite less than 151.49mg/L would increase the nitrogen removal rate, while influent nitrite higher than 170mg/L would inhibited the nitrogen removal rate. The model of Luong suited to fit the inhibition kinetics with higher nitrite concentration which affected nitrogen removal efficiency. The maximum substrate removal rate (NRRmax) obtained by Luong model was 0.53kg N/(m3·d), and the half-saturation constant (KS) was 0.10mg/L. The maximum allowable effluent nitrite concentration above which cells do not grow (Sm) was predicted to be 338.22mg/L and the correlation (n) was 0.97801.
Strous M, Heijnen J, Kuenen J, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms [J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[2]
Dapena-Mora A, Fernández I, Campos J L, et al. Evaluation of activity and inhibition effects on Anammox process by batch tests based on the nitrogen gas production [J]. Enzyme and Microbial Technology, 2007,40(4):859-865.
[3]
Strous M, Kuenen J G, Jetten M S. Key physiology of anaerobic ammonium oxidation [J]. Applied and Environmental Microbiology, 1999,65(7):3248-3250.
[4]
丁 爽.厌氧氨氧化关键技术及其机理的研究 [D]. 杭州:浙江大学, 2014.
[5]
Jetten M S, Strous M, Van De Pas-Schoonen K T, et al. The anaerobic oxidation of ammonium [J]. FEMS Microbiology Reviews, 1998,22(5):421-437.
[6]
Kimura Y, Isaka K, Kazama F, et al. Effects of nitrite inhibition on anaerobic ammonium oxidation [J]. Applied Microbiology and Biotechnology, 2010,86(1):359-365.
[7]
Chen Y, Cheng J J, Creamer K S. Inhibition of anaerobic digestion process: a review [J]. Bioresource Technology, 2008, 99(10):4044-4064.
[8]
Yi Y, Yong H, Huiping D. Effect of salt on Anammox process [J]. Procedia Environmental Sciences, 2011,10,Part C(0):2036-2041.
[9]
Kartal B, Koleva M, Arsov R, et al. Adaptation of a freshwater anammox population to high salinity wastewater [J]. Journal of Biotechnology, 2006,126(4):546-553.
Sheintuch M, Tartakovsky B, Narkis N, et al. Substrate inhibition and multiple states in a continuous nitrification process [J]. Water Research, 1995,29(3):953-963.
[12]
Surmacz-Gorska J, Gernaey K, Demuynck C, et al. Nitrification monitoring in activated sludge by oxygen uptake rate (OUR) measurements [J]. Water Research, 1996,30(5):1228-1236.
Edwards V H. The influence of high substrate concentrations on microbial kinetics [J]. Biotechnology and Bioengineering, 1970, 12(5):679-712.
[16]
Aiba S, Shoda M, Nagatani M. Kinetics of product inhibition in alcohol fermentation [J]. Biotechnology and Bioengineering, 1968,10(6):845-864.
[17]
Luong J H T. Generalization of Monod kinetics for analysis of growth data with substrate inhibition [J]. Biotechnology and Bioengineering, 1987,29(2):242-248.
[18]
Jin R C, Zhang Q Q, Yang G-F, et al. Evaluating the recovery performance of the ANAMMOX process following inhibition by phenol and sulfide [J]. Bioresource Technology, 2013,142:162-170.
[19]
Wang J, Wan W. Kinetic models for fermentative hydrogen production: a review [J]. International Journal of Hydrogen Energy, 2009,34(8):3313-3323.
[20]
Jin R C, Yang G F, Zhang Q Q, et al. The effect of sulfide inhibition on the ANAMMOX process [J]. Water Research, 2013,47(3):1459-1469.
[21]
Yang G F, Zhang Q Q, Jin R C. Changes in the nitrogen removal performance and the properties of granular sludge in an Anammox system under oxytetracycline (OTC) stress [J]. Bioresource Technology, 2013,129:65-71.
Egli K, Fanger U, Alvarez P J J, et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate [J]. Archives of Microbiology, 2001,175(3):198-207.
[27]
Kartal B, Koleva M, Arsov R, et al. Adaptation of a freshwater anammox population to high salinity wastewater [J]. Journal of Biotechnology, 2006,126(4):546-553.
[28]
Wiesmann U. Biological nitrogen removal from wastewater [J]. Biotechnics/Wastewater, 1994:113-154.
[29]
Antileo C, Werner A, Ciudad G, et al. Novel operational strategy for partial nitrification to nitrite in a sequencing batch rotating disk reactor [J]. Biochemical Engineering Journal, 2006,32(2): 69-78.
Jin R C, Ma C, Yu J J. Performance of an Anammox UASB reactor at high load and low ambient temperature [J]. Chemical Engineering Journal, 2013,232(0):17-25.
[32]
Ismail S, De La Parra C, Temmink H, et al. Extracellular polymeric substances (EPS) in upflow anaerobic sludge blanket (UASB) reactors operated under high salinity conditions [J]. Water Research, 2010,44(6):1909-1917.
[33]
Fernández I, Dosta J, Fajardo C, et al. Short and long-term effects of ammonium and nitrite on the Anammox process [J]. Journal of Environmental Management, 2012,95:S170-S174.
[34]
Egli K, Fanger U, Alvarez P J, et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate [J]. Archives of Microbiology, 2001,175(3):198-207.
[35]
Lotti T, Van Der Star W, Kleerebezem R, et al. The effect of nitrite inhibition on the anammox process [J]. Water Research, 2012,46(8):2559-2569.
[36]
Puyol D, Carvajal-Arroyo J, Sierra-Alvarez R, et al. Nitrite (not free nitrous acid) is the main inhibitor of the anammox process at common pH conditions [J]. Biotechnology Letters, 2014,36(3): 547-551.
[37]
Bettazzi E, Caffaz S, Vannini C, et al. Nitrite inhibition and intermediates effects on Anammox bacteria: a batch-scale experimental study [J]. Process Biochemistry, 2010,45(4):573-580.