Rapid determination of the intracellular PHA content during the enhanced biological phosphorus removal using NIR spectroscopy based on the modified PLS methods
XU Ling1,2, LI Wei-hua1,2, YANG Ying1,2, YAN Guo-bing1,2, SHUAI Lei1,2, ZHAO Qing1,2
1. School of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China;
2. Key Laboratory of Anhui Province of Water Pollution Control and Wastewater Reuse, Anhui Jianzhu University, Hefei 230601, China
Poly-β-hydroxyalkanoates (PHA) are vital intracellular storage generated by granular sludge in enhanced biological phosphorus removal (EBPR) reactor, served as carbon and energy source. The rapid determination of PHA is of great significance to the further researches on mechanism of EBPR. Savitzky-Golay smoothing (SG) and Multiplicative scatter correction (MSC) method were jointly used to preprocess the near infrared (NIR) spectra of the sludge samples, the quantitative analysis models between the NIR spectra and the PHA contents were established with modified PLS methods such as interval partial least squares (iPLS), backward interval partial least squares (biPLS) and synergy interval partial least squares (siPLS). SG-MSC pretreatment could effectively diminish the effect of spectrum interference factors. The optimal model was established by four sub-intervals [13,21,24,29] with siPLS method, divided the preprocessed spectrum into 30sub-intervals. The root mean square errors of cross validation (RMSECV) and the root mean square errors of prediction (RMSEP) were 0.2018 and 0.3120 respectively. The correlation coefficients of the calibration and prediction sets were 0.9925 and 0.9391 respectively. The best analysis spectrum band are closely related to the C—H bending vibration and C=O stretching vibration of PHA molecular. The modified PLS could not only optimize the spectra region, enhance the predictive ability of the models, but also realize the rapid and quantitative determination of intracellular PHA content in the granular sludge.
徐玲, 李卫华, 杨英, 严国兵, 帅磊, 赵庆. 基于改进偏最小二乘法的近红外快速分析强化生物除磷污泥胞内PHA[J]. 中国环境科学, 2016, 36(5): 1426-1434.
XU Ling, LI Wei-hua, YANG Ying, YAN Guo-bing, SHUAI Lei, ZHAO Qing. Rapid determination of the intracellular PHA content during the enhanced biological phosphorus removal using NIR spectroscopy based on the modified PLS methods. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(5): 1426-1434.
Mino T, Van Loosdrech M C M, Heijnen J J. Microbiology and biochemistry of the enhanced biological phosphate removal process [J]. Water Research, 1998,32(11):3193-3207.
[2]
Lürling M, Waajen G, Oosterhout F V. Humic substances interfere with phosphate removal by lanthanum modified clay in controlling eutrophication [J]. Water Research, 2014,54(1):78-88.
[3]
Rashed E M, El-Shafei M M, Heikal M A, et al. Application of contact stabilization activated sludge for enhancing biologicalphosphorus removal (EBPR) in domestic wastewater [J]. HBRC Journal, 2014,10(1):92-99.
[4]
Ali H I, Abd El-Azim M M, Abd El-Rahman M S, et al. The effects of modification for contact stabilization activated sludge on EBPR [J]. HBRC Journal, 2015,11(1):143-143.
[5]
Li N, Ren N Q, Wang X H, et al. Effect of temperature on intracellular phosphorus absorption and extracellular phosphorus removal in EBPR process [J]. Bioresource Technology, 2010, 101(15):6265-6268.
Mielczarek A T, Nguyen H T T, Nielsen J L, et al. Population dynamics of bacteria involved in enhanced biological phosphorus removal in Danish wastewater treatment plants [J]. Water Research, 2013,47(4):1529-1544.
[8]
Wong P Y, Cheng K Y, Kaksonen A H, et al. A novel post denitrification configuration for phosphorus recovery using polyphosphate accumulating organisms [J]. Water Research, 2013, 47(17):6488-6495.
[9]
Nielsen P H, Saunders A M, Hansen A A, et al. Microbial communities involved in enhanced biological phosphorus removal from wastewater — A model system in environmental biotechnology [J]. Current Opinion in Biotechnology, 2012, 23(3):452-459.
[10]
Chen Y G, Randall A A, McCue T, et al. The efficiency of enhanced biological phosphorus removal from real wastewater affected by different ratios of acetic topropionic acid [J]. Water Research, 2004,38(1):27-36.
[11]
Fang F, Liu X W, Xu J, et al. Formation of aerobic granules and their PHB production at various substrate and ammonium concentrations [J]. Bioresource Technology, 2009,10(1):59-63.
[12]
Teeka J, Imai T, Reungsang A, et al. Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolated Novosphingobium sp. THA_AIK7using crude glycerol [J]. Society for Industrial Microbiology and Biotechnology, 2012, 39(5):749-758.
[13]
Muhr A, Rechberger M E, Salerno A, et al. Novel Description of mcl-PHA Biosynthesis by Pseudomonaschlororaphis from Animal-Derived Waste [J]. Journal of Biotechnology, 2013, 165(1):45-51.
[14]
Li W H, Mao Q Y, Liu Y X, et al. Analysis of polyhydroxyalkonates (PHA) during the enhanced biological phosphorus removal process using FTIR spectra [J]. Spectroscopy and Spectral Analysis, 2014,34(6):1512-1517.
Majed N, Gu A Z. Application of Raman microscopy for simultaneous and quantitative evaluation of multiple intracellular polymers dynamics functionally relevant to enhanced biological phosphorus removal processes [J]. Environmental Science & Technology, 2010,44(22):8601-8608.
Kang Q, Ru Q G, Xu L Y, et al. On-line monitoring the extract process of Fu-fang Shuanghua oral solution using near infrared spectroscopy and different PLS algorithms [J]. Molecular and Biomolecular Spectroscopy, 2015,152(5):431-437.
[20]
Wang X F, Bao Y F, Liu G L, et al. Study on the Best Analysis Spectral Section of NIR to Detect Alcohol Concentration Based on SiPLS [J]. Procedia Engineering, 2012,29(4):2285-2290.
Chen Q S, Zhao J W, Liu M H, et al. Determination of total polyphenols content in green tea using FT-NIR spectroscopy and different PLS algorithms [J]. Journal of Pharmaceutical and Biomedical Analysis, 2008,46(3):568-573.
Zhang M L, Sheng G P, Mu Y, et al. Rapid and accurate determination of VFAs and ethanol in the effluent of an anaerobic H2-producing bio-reactor using near-infrared spectroscopy Original Research Article [J]. Water Research, 2009,43(7):1823-1830.