1. School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China;
2. Jiangxi Provincial Institute of Water Sciences, Nanchang 330029, China;
3. Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Nanchang 330029, China
Two kinds of mediators, namely syringaldehyde (SA) and1-hydroxybenzotriazole (HBT), were investigated in laccase oxidation system, regarding the degradation of five kinds of typical sulfonamide antibiotics. The influences of factors, such as the initial concentrations of mediators, pH, temperature and the concentration of laccase, were studied. The results showed that SA was more effective in mediating laccase oxidation of all five kinds of sulfonamide antibiotics. In laccase+SA reaction system, the optimum conditions for the degrading the sulfonamide antibiotics by laccase were: at pH5~6 and 30℃, and with initial concentrations of SA and laccase respectively 0.5mmol/L and 0.5mg/mL. The degradation rates reached around 75% in 15minutes and more than 97% within 1hour for all five kinds of sulfonamide antibiotics. This study showed that the appropriate mediator can greatly improve laccase oxidation. Laccase oxidation assisted by mediators has a good application potential for the ecological purification of antibiotic pollutants.
WHO's first global report on antibiotic resistance reveals serious, worldwide threat to public health [R]. WHO, 30APRIL, 2014.
[3]
Sara R. Antibiotic resistance sweeping developing world [J]. Nature, 2014,509:141-142.
[4]
Homem V, Santos L. Degradation and removal methods of antibiotics from aqueous matrices-A review [J]. Journal of Environmental Management, 2011,92:2304-2347.
Christoph A G, Erik M A, Patrick S, et al. Laccases to take on the challenge of emerging organic contaminants in wastewater [J]. Applied Microbiology and Biotechnology, 2014,98:9931-9952.
Shi L, Ma F, Han Y, et al. Removal of sulfonamide antibiotics by oriented immobilized laccase on Fe3O4 nanoparticles with natural mediators [J]. Journal of Hazardous Materials, 2014,279:203- 211.
[9]
Tomoyo S, Takayuki H, Shingo K, et al. Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole [J]. Bioresource Technology, 2012,103:498-501.
[10]
Migliore L, Fiori M, Spadoni A, et al. Biodegradation of oxytetracycline by Pleurotus ostreatus mycelium: a mycoremediation technique [J]. Journal of Hazardous Materials, 2012,215-216:227-232.
[11]
Garcia H A, Hoffman C M, Kinney K A. Laccase-catalyzed oxidation of oxybenzone in municipal wastewater primary effluent [J]. Water research, 45(2011):1921-1932.
[12]
W S, Ku K, Lai H. The implication of mediators for enhancement of laccase oxidation of sulfonamide antibiotics [J]. Bioresource Technology, 113(2012):259-264.
[13]
Nguyen L N, Hai F I, Price W E, et al. The effects of mediator and granular activated carbon addition on degradation of trace organic contaminants by an enzymatic membrane reactor [J]. Bioresource Technology, 2014,167:169-177.
[14]
Canas A I, Camarero S. Laccases and their natural mediators: biotechnological tools for sustainable eco-friendly processes [J]. Biotechnology Advances, 2010,28:694-705.
[15]
Weng S, Ku K, Lai H. The implication of mediators for enhancement of laccase oxidation of sulfonamide antibiotics [J]. Bioresource Technology, 2012,113:259-264.
[16]
靳 蓉,张飞龙.漆酶的结构与催化反应机理 [J]. 中国生漆, 2012,4:6-16.
[17]
张飞龙.中国髹漆工艺与漆器保护 [M]. 北京:科学出版社, 2010.
[18]
Kourosh R, Mohammad A F, Mahvi A H, et al. Elimination and detoxification of sulfathiazole and sulfamethoxazole assisted by laccase immobilized on porous silica beads [J]. International Biodeterioration & Biodegradation, 2015,97:107-114.
[19]
Xu F, Kulys J J, Duke K, et al. Redox chemistry in laccase-catalyzed oxidation of N-hydroxy compounds [J]. Applied and Environmental Microbiology, 2000,5:2052-2056.
