Immobilization and degradation performance of diethylstilbestrol-degrading bacteria S (Serratia sp.)
LING Wan-ting, XU Ran-fang, LIU Juan, SUN Min-xia, LI Shun-yao, ZHU Xue-zhu, GAO Yan-zheng
Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
Diethylstilbestrol (DES)-degrading bacteria S (Serratia sp.) was immobilized using sodium alginate as the embedding agent. The optimum immobilization conditions, determined through orthogonal test, were as follows: 4% sodium alginate, bacteria: sodium alginate 1:2, 4% CaCl2 and 6h crosslinking time. The DES degradation performance of immobilized strain S was apparently superior to that of free strain. The degradation ratio of DES by immobilized strain S was 83.84%, which was 22.84% higher than that by free strain S under laboratory culture conditions. The optimum inoculation of immobilized strain S was 300g/L, and the optimum initial DES concentration was 20~40mg/L. Three water samples were collected from sewage outfalls with DES concentrations of 40.01, 37.90 and 33.52μg/L, respectively. Immobilized strain S was added to these water samples, and 90.0%, 96.0% and 96.7% of DES were removed by immobilized strain S after 7d. Results of this investigation provide a new available technique for estrogen removal from polluted water systems.
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