Diversity, abundance and distribution of nirS-type denitrifiers and Anammox bacteria in sediments of Beiyun River
BAO Lin-lin1,2,3, WANG Xiao-yan1,4, CHEN Yong-juan1, ZHANG Ling-rong1
1. College of Resources, Environment and Tourism, Capital Normal University, Beijing 100048, China;
2. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China;
4. Research Center of Aquatic Environment in the Capital Region, Capital Normal University, Beijing 100048, China
Denitrification and anaerobic ammonia oxidation are two main processes for nitrogen removal in nitrogen cycle. The seasonal variation of community diversity and abundance, phylogenetic composition of nirS-type denitrifiers and Anammox (anaerobic ammonia oxidation) bacteria of sediments in Beiyun River were compared based on PCR (polymerase chain reaction), T-RFLP (terminal restriction fragment length polymorphism), clone and sequencing. The abundance of nirS-type denitrifiers increased from summer to winter while the abundance of Anammox bacteria decreased significantly. What's more, the abundance of nirS-type denitrifiers was significantly higher than Anammox in fall and winter. Community composition of the two microbial groups varied seasonally and the community diversity of nirS-type denitrifiers was much higher than Anammox bacteria. Concentrations of nitrogens and organic carbon in the sediments increased significantly from summer to winter. Environmental temperature was significantly correlated with the seasonal changes of abundance and community distribution of the two microbial groups in sediments. Correlation analysis revealed that total nitrogen had a great effect on the abundance of nirS-type denitrifiers, while C/N was significantly correlated with abundance of Anammox bacteria. NOx- and pH were also the main environmental factors determining the community distribution of nirS-type denitrifiers and Anammox bacteria in sediments. Phylogenetic analysis revealed that most of the denitrifying microbes belonged to species with relatively high pollution-resistance and efficiency of nitrogen removal. Phylogenetic diversity of nirS-type denitrifiers was much higher than that of Anammox bacteria. nirS-type denitrifiers were grouped into genera Pseudomonas and Halomonas, while Anammox was mainly bacteria belonged to Candidatus Brocadia.
鲍林林, 王晓燕, 陈永娟, 张苓荣. 北运河沉积物中主要脱氮功能微生物的群落特征[J]. 中国环境科学, 2016, 36(5): 1520-1529.
BAO Lin-lin, WANG Xiao-yan, CHEN Yong-juan, ZHANG Ling-rong. Diversity, abundance and distribution of nirS-type denitrifiers and Anammox bacteria in sediments of Beiyun River. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(5): 1520-1529.
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