The spectral characteristic and the source of the dissolved organic matter (DOM) were investigated in the treatment of food wastewater with anaerobic sequencing batch reactor (ASBR) through three dimensional fluorescence spectra, and the correlation was analyzed between fluorescence intensity of DOM characteristic peak and ammonia nitrogen concentration. The ASBR results showed that the organic substances can be degraded effectively in food wastewater: the COD concentration in influent and effluent were 1000mg/L and 91mg/L respectively, and the removal rate of COD was 91.73%. There were fluorescence peaks of five substances in three-dimensional fluorescence spectra: high excitation wavelength tryptophan (peak A), low excitation wavelength tryptophan (peak B), visible light wavelength fulvic acid (peak C), UV light wavelength fulvic acid (peak D), and humic acid (peak E). During the reaction, the fluorescence intensities of peak A, peak B and peak C first increased but afterwards decreased, the fluorescence intensity of peak D showed a weak increasing trend, and the fluorescence intensity of peak E first decreased and afterwards increased. Fluorescence spectrum parameters, including fluorescence index (FI), humification index (HIX), and biological index (BIX), indicated that wastewater obviously has biological characteristics. Statistical analyses pointed to correlations between ammonia nitrogen and fluorescence intensity of tryptophan, including fluorescence intensity of peak A, fluorescence intensity of peak B, sum of tryptophan fluorescence intensity, with correlation coefficients being respectively 0.8136, 0.9390 and 0.9153. In the anaerobic biological treatment of food wastewater, rapid detection of ammonia nitrogen can be realized through three-dimensional fluorescence spectrometry.
Henderson R K, Baker A, Murphy K R, et al. Fluorescence as a potential monitoring tool for recycled water system: A review [J]. Water Research, 2009,43(4):863-881.
[3]
Zhang Y L, Yin Y, Feng L Q, et al. Characterizing chromophoric dissolved organic matter in Lake Tianmuhu and its catchment basin using excitation-emission matrix fluorescence and parallel factor analysis [J]. Water Research, 2011,45(16):5110-5122.
[4]
Zhang Y L, Yin Y, Liu X H,et al. Spatial-seasonal dynamics of chromophoric dissolved organic matter in Lake Taihu, a large eutrophic, shallow lake in China [J]. Organic Geochemistry, 2011,42(5):510-519.
Oliveira R P, Ratusznei S M, Rodrigues J A D, et al. Interaction effects of organic load and cycle time in an ASBR applied to a personal care industry wastewater treatment [J]. Journal of environmental management, 2010,91(12):2499-2504.
Baker Andy. Fluorescence properties of some farm wastes: implications for water quality monitoring [J]. Water Research, 2002,36:189-195.
[13]
Yamashita Y, Tanoue E. Chemical characterization of protein-like fluorophores in DOM in relation to aromatic amino acids [J]. Marine Chemistry, 2003,82(3/4):255-271.
[14]
Mayer L M, Schick L L, Loder T C. Dissolved protein fluorescence in two Maine estuaries [J]. Marine Chemistry, 1999,64(3):171-179.
Coble P G. Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy [J]. Marine Chemistry, 1996,51(4):325-346.
Wickland K P, Neff J C, Aiken G R. Dissolved organic carbon in Alaskan boreal forest: Sources, chemical characteristics, and biodegradability [J]. Ecosystems, 2007,10(8):1323-1340.
[19]
Zhang Y L, Zhang E L, Yin Y, et al. Characteristics and sources of chromophoric dissolved organic matter in lakes of Yungui Plateau, China, differing in trophic state and altitude [J]. Limnology and Oceanography, 2010,55(6):2645-2659.
[20]
Huguet A, Vacher L, Relexans S, et al. Properties of fluorescent dissolved organic matter in the Gironde Estuary [J]. Organic Geochemistry, 2009,40(6):706-719.
