Spectroscopic methods study of the interaction of extracellular polymeric substance and tetrabromobisphenol A
BAO Yi-jun, YANG Cun-man, LI Ying, LU Guang-hua
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, HoHai University, Nanjing 210098, China
Three-dimensional fluorescence spectroscopy and infrared spectroscopy were used to investigatethe interaction between tetrabromobisphenol A (TBBA) and extracellular polymeric substance (EPS) from sludge. There were three particular fluorescence peaks in spectra of EPS, Ex/Em=230/300nm (peak A), Ex/Em=240/350nm (peak B) and Ex/Em=270/370nm (peak C), respectively. The results of fluorescence titration revealed that the three fluorescence peaks of EPS could be quenched by TBBA. The binging constants (lg K) were calculated by the modified Stern-Volmer model and the Ryan-Weber model, ranged from 4.23 to 6.27. Infrared spectroscopy and synchronous fluorescence spectroscopy indicated the combination of EPS with TBBA has led to a change in EPS structure. The effect of environmental condition (i.e. pH, ions, and conductivity) on TBBA binding with EPS were evaluated. The results show that pH and ions affected binding affinity, but the effect ofconductivitywas minor.
包宜俊, 杨存满, 李颖, 陆光华. 光谱法研究胞外聚合物与四溴双酚A的相互作用[J]. 中国环境科学, 2016, 36(6): 1773-1779.
BAO Yi-jun, YANG Cun-man, LI Ying, LU Guang-hua. Spectroscopic methods study of the interaction of extracellular polymeric substance and tetrabromobisphenol A. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(6): 1773-1779.
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