Analysis of microbial community structure and functional bacteria in a biofilter with different sequencing technologies
CAI Yan-an1, LI Dong2, BI Xue-jun1, ZENG Hui-ping2, ZHANG Jie2,3
1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
3. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
Effects of the technologies of clone library and high throughout sequencing for revealing microbial communitystructure and functional bacteria in a biofilter which simultaneously removes iron, manganese and ammonia were compared. 15057 sequences with 32 classifications in class level were obtained after the high throughout sequencing process, while there are 9classified groups in clone library. The former revealed more diversity of bacterial community structure. However, in the functional bacteria analysis process, some nitrifiers or iron and manganese bacteria which were detectable in one sequencing analysis process were not appeared in the other process. Comparing with using these two technologies individually in the analysing process, the combined using can compensate for each other and reveal the functional bacteria better.
蔡言安, 李冬, 毕学军, 曾辉平, 张杰. 基于不同测序技术的生物群落结构及功能菌分析[J]. 中国环境科学, 2016, 36(6): 1830-1834.
CAI Yan-an, LI Dong, BI Xue-jun, ZENG Hui-ping, ZHANG Jie. Analysis of microbial community structure and functional bacteria in a biofilter with different sequencing technologies. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(6): 1830-1834.
Regan J M, Harrington G W, Noguera D R. Ammonia-and nitrite-oxidizing bacterial communities in a pilot-scale chloraminated drinking water distribution system [J]. Applied and Environmental Microbiology, 2002,68(1):73-81.
[4]
Siering P, Ghiorse W. Development and application of 16S rRNA-targeted probes for detection of iron-and manganese-oxidizing sheathed bacteria in environmental samples [J]. Applied and Environmental Microbiology, 1997,63(2):644-651.
[5]
Francis C A, Tebo B M. cumA multicopper oxidase genes from diverse Mn(II)-oxidizing and non-Mn(II)-oxidizing Pseudomonas strains [J]. Applied and Environmental Microbiology, 2001,67(9): 4272-4278.
Li X K, Chu Z R, Liu Y J, et al. Molecular characterization of microbial populations in full-scale biofilters treating iron, manganese and ammonia containing groundwater in Harbin, China [J]. Bioresource Technology, 2013(147):234-239.
[8]
Regan J M, Harrington G W, Baribeau H, et al. Diversity of nitrifying bacteria in full-scale chloraminated distribution systems [J]. Water Research, 2003,37(1):197-205.
[9]
Liu T, Li D, Zeng H, et al. Distribution and genetic diversity of functional microorganisms in different CANON reactors [J]. Bioresource Technology, 2012(123):574-580.
[10]
Alawi M, Lipski A, Sanders T, et al. Cultivation of a novel cold-adapted nitrite oxidizing betaproteobacterium from the Siberian Arctic [J]. ISME Journal, 2007,1(3):256-264.
Tekerlekopoulou A G, Vayenas D V. Ammonia, iron and manganese removal from potable water using trickling filters [J]. Desalination, 2007,210(1-3):225-235.
[13]
Michalakos G D, Nieva J M, Vayenas D V, et al. Removal of iron from potable water using a trickling filter [J]. Water Research, 1997,31(5):991-996.
[14]
Hope C K, Bott T R. Laboratory modelling of manganese biofiltration using biofilms of Leptothrix discophora [J]. Water Research, 2004,38(7):1853-1861.
[15]
Burger M S, Krentz C A, Mercer S S, et al. Manganese removal and occurrence of manganese oxidizing bacteria in full-scale biofilters [J]. Journal of Water Supply Research and Technology, 2008,57(5):351-359.
[16]
Carmichael M J, Carmichael S K, Santelli C M, et al. Mn (II)-oxidizing bacteria are abundant and environmentally relevant members of ferromanganese deposits in caves of the upper Tennessee River Basin [J]. Geomicrobiology Journal, 2013,30(9): 779-800.
[17]
Miller A Z, Hernández-Mariné M, Jurado V, et al. Enigmatic reticulated filaments in subsurface granite [J]. Environmental Microbiology Reports, 2012,4(6):596-603.
