In order to improve the ability of peanut shell powders (PS) used as bio-adsorbent to remove heavy metal ions, an isolated Pb²⁺-resistant strain, Aspergillus oryzae (HA), was mixed with peanut shell powders to form a composite adsorbent (PSH). Static and dynamic adsorption experiments were carried out todetermine the adsorption capacity of Pb²⁺ ions onto both PS and PSH in aqueous solutions．The effects of the fixed adsorption bed height, flow rates and initial Pb²⁺ ionconcentration on adsorption behaviors of PSH were investigated. The results from the study indicated that the adsorption capacity of PSH was significantly higher than that of PS. Both the breakthrough time and the height of mass transfer zone increased with increase inbed height and decrease inflow rate and initial concentration. In comparison, the effect of flow rates was significant at higher levels than that of bed height and initial concentration.Thomas model gave satisfactory descriptions on column Pb²⁺ adsorption kinetics (R²>0.95), indicating that neither external nor internal diffusions were the factors limiting the steps ofcolumn adsorption. BDST model could accurately predict the relationship among bed height, c_t/c₀ (the ratio of concentration at a given time c_t to initial concentration c₀) and breakthrough time under different operation conditions. The average deviation error were 2.89 % at c_t/c₀=0.6.