The metabonomics method was employed to investigate the changes in serum metabolic components of Sprague Dawley rats after combined exposure to nonylphenol (NP) and octylphenol (OP). The male rats were randomly divided into control group, low-dose combined exposure group (25mg/kg NP+25mg/kg OP) and high-dose combined exposure group (75mg/kg NP+75mg/kg OP). The rats were treated by gavage once a day for 7 days, and then killed. The metabolic fingerprinting of rat serum was obtained by ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/TOF-MS). Dose-response toxicity was observed based on the principle components analysis (PCA). Meanwhile, the potential biomarkers were screened out according to t-test. The results showed that there was a significant difference in the metabolic profile among the control group, high and low dose groups, and with the increase of exposure dose, the toxicity was enhanced, which showed a dose-effect relationship. Meanwhile, the contents of taurocholic acid and 1-Palmitoyllysophosphatidylcholine increased while the contents of tyrosine, uridine-5'-monophosphate and sulfatide decreased under combined exposure to NP and OP. It is suggested that NP and OP exposure may have adverse effects on the endocrine system, cardiovascular system, nervous system, nucleotide synthesis, sugar and phospholipid metabolism.
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