弱酸性条件下丙酸富集培养物的降解特性

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摘要

为阐明厌氧生物处理系统中pH降低对丙酸降解的影响，考察了弱酸性条件下丙酸富集培养物的降解特征.在污泥接种量为0.22g MLVSS/L，初始丙酸浓度为1000mg/L条件下，对照组（pH7.0）的丙酸能够被该富集培养物快速降解，在接种第6d时，丙酸去除率达到了98.5%.当pH从7.0分别降低至6.5和6.0时，丙酸降解速度立即下降.但经过2~3d的适应后，丙酸降解速率恢复到对照的水平，并分别在培养至第8d和9d时其去除率达到了97%以上.当pH为5.5时，丙酸降解被完全抑制.在整个实验过程中，均未检测到氢气，而乙酸也只有在培养初期有过短暂的积累.该结果表明，在该丙酸富集培养物中，产甲烷菌对弱酸性环境具有更好的耐受性和适应性.

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	张立国
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	李建政

关键词 ：丙酸, 富集培养物, 弱酸性条件, 降解特性

Abstract：

To clarify the effects of pH on propionate degradation in an anaerobic system, the degradation characteristics of a propionate enriched culture at slightly acidic conditions were investigated. Under the biomass of 0.22g MLVSS/L and initial propionate of 1000mg/L conditions, propionate was rapidly oxidized at pH7.0 (control) and propionate removal achieved 98.5% after 6days cultivation. pH decrease, from 7.0 to 6.5 and 6.0, resulted in propionate removal rate was decreased instantaneously. But propionate degradation rate was recovered to the value at pH 7.0 after 2~3 days adaptation. Propionate removal at pH 6.5 and pH 6.0 were reached above 97% after 8 days and 9 days cultivation, respectively. Propionate was hardly decomposed at pH 5.5 during the whole cultivation. During the whole experiment, no hydrogen was detected and acetate was transient accumulation in the early culture. These results indicated methanogens were more acid resistance than propionate-oxidizing bacteria.

Key words： propionate enriched culture slightly acidic conditions degradation characteristics

收稿日期: 2016-04-20

PACS:

X703.5

基金资助:

国家自然科学基金项目（51508316）；山西省基础研究项目（2015021136，2015021134）

通讯作者: 班巧英,讲师,banqiaoying@163.com E-mail: banqiaoying@163.com

作者简介: 张立国(1980-),男,河南安阳人,讲师,博士,主要从事废水厌氧生物处理研究.发表论文12篇.

引用本文:

张立国, 刘建忠, 班巧英, 李建政. 弱酸性条件下丙酸富集培养物的降解特性[J]. 中国环境科学, 2016, 36(12): 3724-3728. ZHANG Li-guo, LIU Jian-zhong, BAN Qiao-ying, LI Jian-zheng. Degradation characteristics of a propionate enriched culture at slightly acidic conditions. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(12): 3724-3728.

链接本文:

http://manu36.magtech.com.cn/Jweb_zghjkx/CN/ 或 http://manu36.magtech.com.cn/Jweb_zghjkx/CN/Y2016/V36/I12/3724

[1]	何强,孙兴福,艾海男,等.两相一体式污泥浓缩消化反应器运行效能及其微生物特性[J].中国环境科学,2012,32(11):2039-2046.
[2]	张立国,李建政,班巧英,等.pH对UASB运行效能及产甲烷互营菌群的影响[J].哈尔滨工业大学学报,2013,45(8):44-49.
[3]	马海玲,郑明月,龚淑芬,等.不同乙酸钠浓度对UASB中颗粒污泥形成的影响[J].环境工程学报,2016,10(3):1065-1069.
[4]	Bai J,Liu H,Yin B,et al.Modeling of enhanced VFAs production from wasteactivated sludge by modified ADM1 with improved particle swarm optimization for parameters estimation[J].Biochemical Engineering Journal,2015,103:22-31.
[5]	王权,蒋建国,张昊巍,等.油脂对餐厨废弃物单相厌氧定向制酸的影响[J].中国环境科学,2015,35(5):1436-1441.
[6]	Dhaked R K,Waghmare C K,Alam S I,et al.Effect of propionate toxicity on methanogenesis of night soil at phychrophilic temperature[J].Bioresource Technology,2003,87:299-303.
[7]	Gallert C,Winter J.Propionic acid accumulation and degradation during restart of a full scale anaerobic biowaste digester[J].Bioresource Technology,2008,99:170-178.
[8]	Pullammanappallil P C,Chynoweth D P,Lyberatos G,et al.Stable performance of anaerobic digestion in the presence of a high concentration of propionic acid[J].Bioresource Technology,2001,78:165-169.
[9]	Ban Q,Li J,Zhang L,Jha A K,et al.Microbialcommunity composition and response to temperature shock of a mesophilic propionate-degrading methanogenic consortium[J].International Journal of Agriculture and Biology,2013,15:915-920.
[10]	Worm P,Stams A J M,Cheng X,et al.Growth-and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei[J].Microbiology,2011,157:280-289.
[11]	徐恒,汪翠萍,颜锟,等.颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究[J].中国环境科学,2016,36(5):1435-1441.
[12]	Rotaru A E,Shrestha P M,Liu F,et al.A new model for electron flow during anaerobic digestion:direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J].Energy&Environmental Science,2014,7(1):408-415.
[13]	Li H,Chang J,Liu P,et al.Direct interspecies electron transfer accelerates syntrophic oxidation of butyrate in paddy soil enrichments[J].Environmental Microbiology,2015,17(5):1533-1547.
[14]	高靖伟,赫婷婷,程翔,等.EGSB-MBR组合工艺处理糖蜜发酵废水效能研究[J].中国环境科学,2015,35(5):1416-1422.
[15]	Li J,Ban Q,Zhang L,et al.Syntrophic propionate degradation in anaerobic digestion:a review[J].International Journal of Agriculture and Biology,2012,14(5):843-850.
[16]	国家环保局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].北京:中国环境科学出版社,2002.
[17]	Owen W F,Stuckey D C,Healy J B,et al.Bioassay for monitoring biochemical methane potential and anaerobic toxicity[J].Water Research,1979,13:485-492.
[18]	Wallrabenstein C,Hauschild E,Schink B.Syntrophobacter pfennigii sp.nov.,new syntrophically propionate-oxidizing anaerobe growing in pure culture with propionate and sulfate[J].Archives of Microbiology,1995,164:346-352.
[19]	Harmsen H J M,van Kuijk B L M,Plugge C M,et al.Syntrophobacter fumaroxidans sp.nov.,a syntrophic propionate-degrading sulfate-reducing bacterium[J].International Journal of Systematic Bacteriology,1998,48:1383-1387.
[20]	Liu Y,Whitman W B.Metabolic,phylogenetic,and ecological diversity of the methanogenic archaea[J].Annals of the New York Academy of Sciences,2008,1125:171-189.
[21]	Mu Y,Wang G,Yu H,Response surface methodological analysis on biohydrogen production by enriched anaerobic cultures[J].Enzyme and Microbial Technology,2006,38:905-913.
[22]	Ban Q,Li J,Zhang L,et al.Phylogenetic Diversity of methanogenic archaea and kinetics of methane production at slightly acidic conditions of an anaerobic sludge[J].International Journal of Agriculture and biology,2013,15:347-351.
[23]	Kim I S,Hwang M H,Jang N J,et al.Effect of low pH on the activity of hydrogen utilizing methanogen in bio-hydrogen process[J].International Journal of Hydrogen Energy,2004,29:1133-1140.
[24]	Chen X,Sun Y,Xiu Z,et al.2006.Stoichiometric analysis of biological hydrogen production by fermentative bacteria[J].International Journal of Hydrogen Energy,2006,31:539-549.
[1]	何强,孙兴福,艾海男,等.两相一体式污泥浓缩消化反应器运行效能及其微生物特性[J].中国环境科学,2012,32(11):2039-2046.
[2]	张立国,李建政,班巧英,等.pH对UASB运行效能及产甲烷互营菌群的影响[J].哈尔滨工业大学学报,2013,45(8):44-49.
[3]	马海玲,郑明月,龚淑芬,等.不同乙酸钠浓度对UASB中颗粒污泥形成的影响[J].环境工程学报,2016,10(3):1065-1069.
[4]	Bai J,Liu H,Yin B,et al.Modeling of enhanced VFAs production from wasteactivated sludge by modified ADM1 with improved particle swarm optimization for parameters estimation[J].Biochemical Engineering Journal,2015,103:22-31.
[5]	王权,蒋建国,张昊巍,等.油脂对餐厨废弃物单相厌氧定向制酸的影响[J].中国环境科学,2015,35(5):1436-1441.
[6]	Dhaked R K,Waghmare C K,Alam S I,et al.Effect of propionate toxicity on methanogenesis of night soil at phychrophilic temperature[J].Bioresource Technology,2003,87:299-303.
[7]	Gallert C,Winter J.Propionic acid accumulation and degradation during restart of a full scale anaerobic biowaste digester[J].Bioresource Technology,2008,99:170-178.
[8]	Pullammanappallil P C,Chynoweth D P,Lyberatos G,et al.Stable performance of anaerobic digestion in the presence of a high concentration of propionic acid[J].Bioresource Technology,2001,78:165-169.
[9]	Ban Q,Li J,Zhang L,Jha A K,et al.Microbialcommunity composition and response to temperature shock of a mesophilic propionate-degrading methanogenic consortium[J].International Journal of Agriculture and Biology,2013,15:915-920.
[10]	Worm P,Stams A J M,Cheng X,et al.Growth-and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei[J].Microbiology,2011,157:280-289.
[11]	徐恒,汪翠萍,颜锟,等.颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究[J].中国环境科学,2016,36(5):1435-1441.
[12]	Rotaru A E,Shrestha P M,Liu F,et al.A new model for electron flow during anaerobic digestion:direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J].Energy&Environmental Science,2014,7(1):408-415.
[13]	Li H,Chang J,Liu P,et al.Direct interspecies electron transfer accelerates syntrophic oxidation of butyrate in paddy soil enrichments[J].Environmental Microbiology,2015,17(5):1533-1547.
[14]	高靖伟,赫婷婷,程翔,等.EGSB-MBR组合工艺处理糖蜜发酵废水效能研究[J].中国环境科学,2015,35(5):1416-1422.
[15]	Li J,Ban Q,Zhang L,et al.Syntrophic propionate degradation in anaerobic digestion:a review[J].International Journal of Agriculture and Biology,2012,14(5):843-850.
[16]	国家环保局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].北京:中国环境科学出版社,2002.
[17]	Owen W F,Stuckey D C,Healy J B,et al.Bioassay for monitoring biochemical methane potential and anaerobic toxicity[J].Water Research,1979,13:485-492.
[18]	Wallrabenstein C,Hauschild E,Schink B.Syntrophobacter pfennigii sp.nov.,new syntrophically propionate-oxidizing anaerobe growing in pure culture with propionate and sulfate[J].Archives of Microbiology,1995,164:346-352.
[19]	Harmsen H J M,van Kuijk B L M,Plugge C M,et al.Syntrophobacter fumaroxidans sp.nov.,a syntrophic propionate-degrading sulfate-reducing bacterium[J].International Journal of Systematic Bacteriology,1998,48:1383-1387.
[20]	Liu Y,Whitman W B.Metabolic,phylogenetic,and ecological diversity of the methanogenic archaea[J].Annals of the New York Academy of Sciences,2008,1125:171-189.
[21]	Mu Y,Wang G,Yu H,Response surface methodological analysis on biohydrogen production by enriched anaerobic cultures[J].Enzyme and Microbial Technology,2006,38:905-913.
[22]	Ban Q,Li J,Zhang L,et al.Phylogenetic Diversity of methanogenic archaea and kinetics of methane production at slightly acidic conditions of an anaerobic sludge[J].International Journal of Agriculture and biology,2013,15:347-351.
[23]	Kim I S,Hwang M H,Jang N J,et al.Effect of low pH on the activity of hydrogen utilizing methanogen in bio-hydrogen process[J].International Journal of Hydrogen Energy,2004,29:1133-1140.
[24]	Chen X,Sun Y,Xiu Z,et al.2006.Stoichiometric analysis of biological hydrogen production by fermentative bacteria[J].International Journal of Hydrogen Energy,2006,31:539-549.