Cu<sup>2+</sup>对铜绿微囊藻生长及叶绿素荧光主要参数的影响研究

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摘要

研究了不同Cu²⁺浓度（0.16，0.32，0.66，1.16，2.16，4.16μmol/L）对培养基中铜绿微囊藻120h内的细胞密度、叶绿素a含量和3个主要叶绿素荧光参数的影响，并对Cu²⁺在灭藻时易复发的问题做出了一定解释.实验结果表明，当培养体系中Cu²⁺浓度在0.32~1.16 μmol/L时，可促进铜绿微囊藻生长；当Cu²⁺浓度低至0.16μmol/L或高于2.16μmol/L时，可抑制铜绿微囊藻生长.当培养体系中Cu²⁺浓度小于2.16μmol/L时，对Fv/Fm（光系统Ⅱ的最大光能转化效率）、ΦPSⅡ（光系统Ⅱ的实际光能转化效率）和ETR（电子传递效率）3个荧光参数指标的影响不大；当Cu²⁺浓度为4.16μmol/L时，Fv/Fm、ΦPSⅡ和ETR呈现先快速下降，后有所回升，再缓慢下降的趋势，但整个时段均显著低于其他各浓度处理组（P<0.05），表现出明显的受抑制现象.

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	王寿兵
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关键词 ：铜绿微囊藻, 硫酸铜, 浓度, 生长, 叶绿素荧光

Abstract：

The effects of different Cu²⁺ concentrations (0.16, 0.32, 0.66, 1.16, 2.16, 4.16μmol/L) in culture media on the cell density, chlorophyll-a content and chlorophyll fluorescence parameters (CFP) of Microcystis aeruginosa within the first 120h were investigated. It shows that Cu²⁺ concentration had significant effects on the growth and chlorophyll-a content of Microcystis aeruginosa (P<0.05) from 12h to 120h by One-way ANOVA. The growth of Microcystis aeruginosa could be promoted as the Cu²⁺ concentration was between 0.32~1.16μmol/L and can be inhibited as that is below 0.16μmol/L or above 2.16μmol/L. The obvious effects on the CFPs of Fv/Fm, ΦPSⅡ and ETR were not observed when the Cu²⁺ concentration is below 2.16μmol/L. But the serious bad influences were found when the Cu²⁺ concentration was 4.16μmol/L. Its values of Fv/Fm, ΦPSⅡ and ETR were much less than that of other Cu²⁺ concentration (P<0.05), while shows the following changing trends:rapidly decreasing within the first 24h, then rapidly increasing within 24h~96h and finally slowly decreasing within 96h~120h.

Key words： Microcystis aeruginosa copper concentration growth chlorophyll fluorescence

收稿日期: 2016-04-15

PACS:

X171

基金资助:

国家水体污染控制与治理科技重大专项（2012ZX07102-004）

通讯作者: 王寿兵,教授,sbwang@fudan.edu.cn E-mail: sbwang@fudan.edu.cn

作者简介: 王寿兵(1970-)男,四川富顺人,教授,博士,主要研究方向为产业生态学和湖泊生态修复.发表论文70余篇.

引用本文:

王寿兵, 徐紫然, 马小雪, 樊正球, 张洁. Cu²⁺对铜绿微囊藻生长及叶绿素荧光主要参数的影响研究[J]. 中国环境科学, 2016, 36(12): 3759-3765. WANG Shou-bing, XU Zi-ran, MA Xiao-xue, FAN Zheng-qiu, ZHANG Jie. Effects of Cu²⁺ on the growth and main parameters of chlorophyll fluorescence of Microcystis aeruginosa. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(12): 3759-3765.

链接本文:

http://manu36.magtech.com.cn/Jweb_zghjkx/CN/ 或 http://manu36.magtech.com.cn/Jweb_zghjkx/CN/Y2016/V36/I12/3759

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[31]	Juneau P,Dewez D,Matsui S,et al.Evaluation of different algal species sensitivity to mercury and metolachlor by PAM-fluorometry[J].Chemosphere,2001,45(4/5):589-598.
[1]	Codd G A.Cyanobacterial toxins,the perception of water quality,and the prioritisation of eutrophication control[J].Ecological Engineering,2000,16(1):51-60.
[2]	Sinclair J L,Hall S.Chapter 3:Occurrence of cyanobacterial harmful algal blooms workgroup report[J].Advances in Experimental Medicine and Biology,2008,619:45-103.
[3]	Carmichael W W.Health effects of toxin-producing cyanobacteria:"The CyanoHABs"[J].Human and Ecological Risk Assessment,2001,7(5):1393-1407.
[4]	彭桂莹,陈永玲,韩玉珍,等.乳酸对铜绿微囊藻的抑藻效应及机理[J].中国环境科学,2016,36(4):1167-1172.
[5]	Wang Z C,Li D H,Qin H J,et al.An integrated method for removal of harmful cyanobacterial blooms in eutrophic lakes[J].Environmental Pollution,2012,160:34-41.
[6]	储昭升,庞燕,郑朔芳,等.超声波控藻及对水生生态安全的影响[J].环境科学学报,2008(7):1335-1339.
[7]	WHO.Guidlines for Drinking water Quality.In health criteria and other supporting information[M].2ed.Geneva:World Heaith Organization,1996.
[8]	刘建康.高级水生生物学[M].北京:科学出版社,2002.
[9]	任撑住,李进,姜兰,等.络合铜对藻类、车轮虫的杀灭试验及对鳜鱼的安全性试验[J].中国兽药杂志,2004,38(9):14-16.
[10]	赵小丽,宋立荣,张小明.硫酸铜控藻对浮游植物群落的影响[J].水生生物学报,2009,33(4):596-602.
[11]	杨弯弯,武氏秋贤,吴亦潇,等.恩诺沙星和硫氰酸红霉素对铜绿微囊藻的毒性研究[J].中国环境科学,2013,33(10):1829-1834.
[12]	Ou H,Gao N Y,Deng Y,et al.Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity,survival and microcystin-LR release risk of Microcystis aeruginosa[J].Water Research,2012,46(4):1241-1250.
[13]	Pena-Vazquez E,Perez-Conde C,Costas E,et al.Development of a microalgal PAM test method for Cu (Ⅱ) in waters:comparison of using spectrofluorometry[J].Ecotoxicology,2010,19(6):1059-1065.
[14]	Wang S Z,Chen F L,Mu S Y,et al.Simultaneous analysis of photosystem responses of Microcystis aeruginoga under chromium stress[J].Ecotoxicology and Environmental Safety,2013,88:163-168.
[15]	王帅,梁英,冯力霞,等.重金属胁迫对杜氏盐藻生长及叶绿素荧光特性的影响[J].海洋科学,2010,(10):38-48.
[16]	陈雷.重金属胁迫对斜生栅藻和铜绿微囊藻生长及叶绿素荧光特性研究[D].南京:南京农业大学,2009.
[17]	邓春暖,叶进霞.高钙环境下蓝藻对铜胁迫的叶绿素荧光响应研究[J/OL].中国科技论文在线,http://www.paper.edu.cn,2014.
[18]	Zhou S Q,Shao Y S,Gao N Y,et al.Effects of different algaecides on the photosynthetic capacity,cell integrity and microcystin-LR release of Microcystis aeruginosa[J].Science of the Total Environment,2013,463:111-119.
[19]	国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,670-671.
[20]	张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[21]	Demming-Adams B,Adams W W,Barker D H,et al.Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physio.Plant.,1996,98:253-264.
[22]	姜恒,吴斌,阎冰,等.微藻叶绿素荧光技术在环境监测中的应用[J].环境工程技术学报,2012,2(2):172-178.
[23]	Kumar K S,Dahms H U,Lee J S,et al.Algal photosynthetic responses to toxic metals and herbicides assessed by chlorophyll a fluorescence[J].Ecotoxicology and Environmental Safety,2014,104:51-71.
[24]	Maxwell K,Johnson G N.Chlorophyll fluorescence-a practical guide[J].Journal of Experimental Botany,2000,51(345):659-668.
[25]	Murchie E H,Lawson T.Chlorophyll fluorescence analysis:a guide to good practice and understanding some new applications[J].Journal of Experimental Botany,2013,64(13):3983-3998.
[26]	Genty B,Briantais J M,Baker N R.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochim.Biophys.Acta.,1989,990:87-92.
[27]	Björkman O,Demmig B.Photon yield of O2 evolution and chlorophyll fluorescence at 77k among vascular plants of divere origins[J].Planta,1987,170:489-504.
[28]	Chaloub R M,Reinert F,Nassar C A G,et al.Photosynthetic properties of three Brazilian seaweeds[J].Revis.Brasil.Bot.,2010,33(2):371-374.
[29]	Kooten O V,Snel J F H.The use of chlorophyll fluorescence nomenclature in plant stress physiology[J].Photosynthesis Research,1990,25:147-150.
[30]	Bilger W,Björkman O.Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes,fluorescence and photosynthesis in leaves of Hedera canariensis[J].Photosynthesis Research,1990,25:173-185.
[31]	Juneau P,Dewez D,Matsui S,et al.Evaluation of different algal species sensitivity to mercury and metolachlor by PAM-fluorometry[J].Chemosphere,2001,45(4/5):589-598.