Effects of the phytoplankton-derived particulate organic matter on the growth and phosphorus enrichment of phosphorus-deficiency Microcystis aeruginosa
HE Dong1,2, CHAO Jian-ying1,3, ZHANG Yi-min1, YANG Fei1, WANG Yu1, GUO Yan-ming1
1. Nanjing Institute of Environmental Science, Ministry of Environmental Protection, Nanjing 210042, China; 2. Jiangsu Academy of Environmental Industry and Technology Corporation, Ltd, Nanjing 210029, China; 3. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China
Abstract:The phytoplankton-derived particulate organic matter (POM) in Lake Taihu was taken as the breakthrough point in this study which referenced phosphorus in the orthophosphate (K2HPO4), polyphosphate (Na5P3O10), orthophosphate monoester (G-6-P) and the orthophosphate diester (lecithin). The effects of phosphorus in different forms on the growth and phosphorus enrichment of phosphorus-deficiency M. aeruginosa was studied. Results showed that the phosphorus-deficiency M. aeruginosa with density of (1.9±0.1)×106cells/mL cannot directly use Na5P3O10 and lecithin whose short-term enrichment were 2.034mg/L, 1.030mg/L. It was significantly higher than the 0.491mg/L of K2HPO4, the 0.034mg/L of G-6-P and the 0.573mg/L of phytoplankton-derived particulate organic matter (POM). Maybe these forms of P cannot enter the cells and were accumulated between the cell membrane and cell wall. M. aeruginosa can use the phytoplankton-derived POM rapidly by releasing alkaline phosphatase enzymes. The growth rate of M. aeruginosa with phytoplankton-derived POM was 0.148d-1 and was slightly lower than the 0.156d-1of K2HPO4, but it was much higher than the rate of other groups. The utilization rate of phytoplankton-derived POM in the end of the experiment was 57.8% which was higher than the 32.5% of K2HPO4 and the 24.4% of lecithin. The composition of phosphorus in phytoplankton-derived POM were mainly orthophosphate and monoester by using liquid-phase NMR analysis. And we verified the effects of it on the growth and phosphorus enrichment of phosphorus-deficiency M. aeruginosa by calculation. In conclusion, the phytoplankton-derived POM had a strong bioavailability, and was very important to the outbreak and persistence on algae bloom.
何东, 晁建颖, 张毅敏, 杨飞, 王宇, 郭艳敏. 藻源性颗粒有机物对磷饥饿微囊藻磷富集与生长的影响[J]. 中国环境科学, 2016, 36(12): 3777-3783.
HE Dong, CHAO Jian-ying, ZHANG Yi-min, YANG Fei, WANG Yu, GUO Yan-ming. Effects of the phytoplankton-derived particulate organic matter on the growth and phosphorus enrichment of phosphorus-deficiency Microcystis aeruginosa. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(12): 3777-3783.
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