For the purpose of enhancing nitrogen removal efficiency of the high-ammonium fertilizer wastewater, an intermittently-aerated sequencing batch reactor (IASBR) and a continuously-aerated sequencing batch reactor (CASBR) were developed to investigate the performance of partial nitrification for fertilizer wastewater treatment at room temperature (16~26℃). In the steady state, more than 90% of COD was removed. The mean NH+ 4-N removal rate was 93.8% and 87.2%, and the mean nitrite accumulation rate reached at 72.6% and 65.6% in IASBR and CASBR, respectively. Partial nitrification kinetics revealed that the ratio of NH4+-N and NO2--N maximum specific nitrification rates were up to 830:1 and 16:1 in IASBR and CASBR, respectively. In order to analyze the improvement effects of intermittent aeration on the performance of partial nitrification, a series of experiments with different non-aeration durations were conducted. It showed that the optimal NO2--N accumulation efficiency appeared at 20min non-aeration/40min aeration condition. The non-aeration phase of intermittent aeration was able to enhance the activity expression of AOB, and induce the efficient NH4+-N removal and NO2--N accumulation. Moreover, the free ammonia concentration in SBRs (1.3~7.5mg/L) was also one of key parameters for achieving partial nitrification.
Song Y J, Ishii S, Rathnayake L, et al. Development and characterization of the partial nitrification aerobic granules in a sequencing batch airlift reactor[J]. Bioresource Technology, 2013,139(13):285-291.
[2]
Wei D, Xue X, Yan L, et al. Effect of influent ammonium concentration on the shift of full nitritation to partial nitrification in a sequencing batch reactor at ambient temperature[J]. Chemical Engineering Journal, 2014,235(1):19-26.
[3]
Kornaros M, Dokianakis S N, Lyberatos G. Partial nitrification/denitrification can be attributed to periodic anoxic disturbances[J]. Environmental Science & Technology, 2010, 44(19):7245-7253.
[4]
Ge S, Wang S, Yang X, et al. Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment:A review[J]. Chemosphere, 2015,140:85-98.
[5]
Rongsayamanont C, Limpiyakorn T, Khan E. Effects of inoculum type and bulk dissolved oxygen concentration on achieving partial nitrification by entrapped-cell-based reactors[J]. Bioresource Technology, 2014,164(7):254-263.
[6]
Xue Y, Yang F, Liu S, et al. The influence of controlling factors on the start-up and operation for partial nitrification in membrane bioreactor[J]. Bioresource Technology, 2009,100(3):1055-1060.
[7]
Lochmatter S, Gonzalez-Gil G, Holliger C. Optimized aeration strategies for nitrogen and phosphorus removal with aerobic granular sludge[J]. Water Research, 2013,47(16):6187-6197.
[8]
Capodici M, Bella G D, Trapani D D, et al. Pilot scale experiment with MBR operated in intermittent aeration condition:Analysis of biological performance[J]. Bioresource Technology, 2015,177:398-405.
Ford D L, Churchwell R L, Kachtick J W. Comprehensive analysis of nitrification of chemical processing wastewaters[J]. Journal Water Pollution Control Federation, 1980,52(11):2726- 2746.
Monod J. The growth of bacterial cultures[J]. Annual Review of Microbiology, 1949,3:371-394.
[15]
Henze M, Gujer W, Mino T, et al. Activated sludge models ASM1, ASM2, ASM2d and ASM3[R]. London:IWA Publishing, 2000.
[16]
Guadie A, Xia S, Zhang Z, et al. Effect of intermittent aeration cycle on nutrient removal and microbial community in a fluidized bed reactor-membrane bioreactor combo system[J]. Bioresource Technology, 2014,156(4):195-205.
[17]
Garrido J M, van Benthum W A J, van Loosdercht M C M, et al. Influence of dissolved oxygen concentration on nitrite accumulation in a biofilm airlift suspension reactor[J]. Biotechnology & Bioengineering, 1997,53(2):168-178.
[18]
Elawwad A, Sandner H, Kappelmeyer U, et al. Long-term starvation and subsequent recovery of nitrifiers in aerated submerged fixed-bed biofilm reactors[J]. Environmental Technology, 2013,34(8):945-959.
Yu R, Chandran K. Strategies of Nitrosomonas europaea 19718to counter low dissolved oxygen and high nitrite concentrations[J]. BMC Microbiology, 2010,10(1):70.
[21]
Wei X, Yan T, Hommes N G, et al. Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate[J]. FEMS Microbiology Letters, 2006,257(1):76-83.
[22]
Hao X D, Wang Q L, Zhang X P, et al. Experimental evaluation of decrease in bacterial activity due to cell death and activity decay in activated sludge[J]. Water Research, 2009,43(14):3604-3612.
[23]
Kim Y H, Yoo C, Lee I B. Optimization of biological nutrient removal in a SBR using simulation-based iterative dynamic programming[J]. Chemical Engineering Journal, 2008,139(1):11-19.
[24]
Chachuat B, Roche N, Latifi M A. Optimal aeration control of industrial alternating activated sludge plants[J]. Biochemical Engineering Journal, 2005,23(3):277-289.
[25]
Holenda B, Domokos E, Redey A, et al. Aeration optimization of a wastewater treatment plant using genetic algorithm[J]. Optimal Control Applications and Methods, 2007,28(3):191-208.
[26]
Souza S M, Araujo O Q F, Coelho M A Z. Model-based optimization of a sequencing batch reactor for biological nitrogen removal[J]. Bioresource Technology, 2008,99(8):3213-3223.
[27]
Zhang M, Lawlor P G, Wu G, et al. Partial nitrification and nutrient removal in intermittently aerated sequencing batch reactors treating separated digestate liquid after anaerobic digestion of pig manure[J]. Bioprocess and Biosystems Engineering, 2011,34(9):1049-1056.
[28]
Sinha B, Annachhatre A P. Partial nitrification-operational parameters and microorganisms involved[J]. Reviews in Environmental Science and Bio-Technology, 2007,6(4):285-313.
[29]
Kim D J, Lee D I, Keller J. Effect of temperature and free ammonia on nitrification and nitrite accumulation in landfill leachate and analysis of its nitrifying bacterial community by FISH[J]. Bioresource Technology, 2006,97(3):459-468.
[30]
Anthonisen A C, Loehr R C, Prakasam T B S, et al. Inhibition of nitrification by ammonia and nitrous acid[J]. Journal Water Pollution Control Federation, 1976,48(5):835-852.
[31]
Pambrun V, Paul E, Sperandio M. Modeling the partial nitrification in sequencing batch reactor for biomass adapted to high ammonia concentrations[J]. Biotechnology & Bioengineering, 2006,95(1):120-131.
