High-throughput profiling of antibiotic resistance genes in river in the vicinity of a landfill
HUANG Fu-yi1, AN Xin-li1,2, LI Li3, OUYANG Wei-ying1,2, LI Hu1,2, SU Jian-qiang1
1. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. Lijiang Environmental Monitoring Station, Lijiang 674100, China
Aquatic environment especially the river is an important reservoir of antibiotic resistance genes (ARGs). For the further insight into the ARGs in a river in the vicinity of a landfill, high-throughput qPCR technique was used to investigate the diversity and abundance of ARGs. The results showed that a total of 57ARGs was detected in control sample, while, 158ARGs was detected in the downstream river sample, in which 150ARGs in the downstream river were significantly enriched (P<0.05). A maximum enrichment of 2299-fold was detected in dfrA1, which conferred resistance to sulfonamide. The detection number of ARGs and the enrichment of ARGs were significantly increased along the river. The abundance of mobile genetic elements was significantly correlated with the abundance of ARGs. These results implied that landfill may lead to the dissemination and enrichment of ARGs in river.
黄福义, 安新丽, 李力, 欧阳纬莹, 李虎, 苏建强. 生活垃圾填埋场对河流抗生素抗性基因的影响[J]. 中国环境科学, 2017, 37(1): 203-209.
HUANG Fu-yi, AN Xin-li, LI Li, OUYANG Wei-ying, LI Hu, SU Jian-qiang. High-throughput profiling of antibiotic resistance genes in river in the vicinity of a landfill. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(1): 203-209.
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