基于响应面法优化一株低温耐盐芘降解菌共代谢条件的研究

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摘要

为了提高低温耐盐芘降解菌DYC-1的共代谢降解率，在已获悉菌株降解最优pH值、初始浓度、摇床转速和接菌量的情况下，利用Plackett-Burman实验设计筛选出影响菌株DYC-1降解PAHs芘的3个外加碳源显著影响因子为葡萄糖、水杨酸和菲，用最陡爬坡实验逼近3个因子的最大响应区域，采用Box-Behnken实验设计及响应面法分析，确定其最优共代谢条件为：葡萄糖225.83mg/L，水杨酸112.10mg/L，菲198.06mg/L；在此条件下培养10d，芘降解率可达到50.69%，相对于不加共代谢底物时提升了23.14%.表明响应面法对菌株共代谢条件的优化合理可行.

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	刁硕
	王红旗
	吴枭雄
	赵一村

关键词 ：响应面法, 共代谢, 优化, 降解菌

Abstract：

In order to enhance pyrene bacteria cometabolism degradation rate under low temperature and high salt environment, under the circumstances of learned optimal pH, initial concentration of pyrene, shaking rates of rocking bed, inoculating dosage, Plackett-Burman Design was conducted and glucose, salicylic acid and phenanthrene were identified as main factors. These factors were approached to optimal region by steepest ascent design. Then optimal degradation condition(glucose, 225.83mg/L; salicylic acid, 112.10mg/L; phenanthrene, 198.06mg/L) was established by Box-Behnken design and reponse surface analysis. Degradation rate under the optimal consition was 50.69% after 10days, enhanced 23.14% compare to the condition of without cometabolism degradation substrate. Experimental results also showed that the method of optimization for bacteria cometabolism degradation is reasonable and feadible.

Key words： response surface methodology(RSM) cometabolism optimization degradation bacteria

收稿日期: 2016-03-21

PACS:

X172

基金资助:

国家自然科学基金面上项目（41372232）；国家“863”项目（2013AA06A205）；中国博士后科学基金第54批面上资助项目

通讯作者: 王红旗,教授,whongqi310@sohu.com E-mail: whongqi310@sohu.com

作者简介: 刁硕(1992-),女,山东荷泽人,北京师范大学水科学研究院硕士研究生,从事污染土壤修复与治理研究.

引用本文:

刁硕, 王红旗, 吴枭雄, 赵一村. 基于响应面法优化一株低温耐盐芘降解菌共代谢条件的研究[J]. 中国环境科学, 2017, 37(1): 345-351. DIAO Shuo, WANG Hong-qi, WU Xiao-xiong, ZHAO Yi-cun. Optimization for pyrene bacteria cometabolism degradation under the low temperature and high salt environment through response surface. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(1): 345-351.

链接本文:

http://manu36.magtech.com.cn/Jweb_zghjkx/CN/ 或 http://manu36.magtech.com.cn/Jweb_zghjkx/CN/Y2017/V37/I1/345

[1]	巩宗强,李培军,王新,等.芘在土壤中的共代谢降解研究[J].应用生态学报,2001,12(3):447-450.
[2]	Heitkamp M A,Cerniglia C E.Polycyclic aromatic hydrocarbon degradation by a Mycobacterium sp.in microcosms containing sediment and water from a pristine ecosystem.[J].Applied&Environmental Microbiology,1989,55(8):1968-1973.
[3]	Bossert I D,Bartha R.Structure-biodegradability relationships of polycyclic aromatic hydrocarbons in soil[J].Bulletin of Environmental Contamination and Toxicology,1986,37(1):490-495.
[4]	孙凯,刘娟,李欣,等.两株具有芘降解功能的植物内生细菌的分离筛选及其特性[J].生态学报,2014,34(4):853-861.
[5]	段燕青,岳秀萍,白凡玉,等.耐受重金属的芘降解真菌的筛选及芘降解动力学研究[J].环境工程学报,2015,9(2):977-982.
[6]	Patel J G,Kumar J I N,Kumar R N,et al.Enhancement of pyrene degradation efficacy of sp.by construction of an artificial microalgal-bacterial consortium[J].Cogent Chemistry,2015,1(1):1064193.
[7]	Obayori O S,Ilori M O,Adebusoye S A,et al.Pyrene-degradation potentials of Pseudomonas species isolated from polluted tropical soils[J].World Journal of Microbiology and Biotechnology,2008,24(11):2639-2646.
[8]	王晓蓉.环境化学[M].南京:南京大学出版社,1997.
[9]	鄢恒珍,龚文琪,梅光军,等.共代谢条件下丁基黄药的生物降解实验研究[J].环境污染与防治,2010,32(4):1-5.
[10]	王欣,王金翠,殷晓梅,等.乙酰甲胺磷UV-TiO2/类Fenton光催化降解过程的响应面法优化[J].应用化工,2013,42(1):33-40.
[11]	尹亮.混合菌群共培养对偶氮染料的协同脱色及降解的研究[D].广州:华南理工大学,2009.
[12]	陈晓鹏,易筱筠,陶雪琴,等.石油污染土壤中芘高效降解菌群的筛选及降解特性研究[J].环境工程学报,2008,2(3):413-417.
[13]	张宏波,林爱军,刘爽,等.芘高效降解菌的分离鉴定及其降解特性研究[J].环境科学,2010,31(1):243-248.
[14]	唐玉斌,马姗姗,王晓朝,等.一株芘的高效降解菌的选育及其降解性能研究[J].环境工程学报,2011,5(1):48-54.
[15]	张娜,尹华,彭辉,等.嗜麦芽窄食单胞菌J12对芘的降解特性[J].环境科学与技术,2010,33(4):6-9.
[16]	温继伟,高大文.偏肿拟栓菌共代谢降解芘条件的优化[J].中国环境科学,2011,31(9):1516-1522.
[17]	钟鸣,张佳庆,吴小霞,等.芘高效降解菌的分离鉴定及其降解特性[J].应用生态学报,2010,21(5):1334-1338.
[18]	王蕾,聂麦茜,王志盈,等.PAHs降解菌的筛选及其对芘的降解研究[J].环境科学与技术,2010,33(1):43-47.
[19]	胡凤钗,苏振成,孙健,等.高效芘降解菌N12的分离鉴定与降解特性[J].应用生态学报,2011,22(6):1566-1572.
[20]	Reddy L V A,Wee Y J,Yun J S,et al.Optimization of alkaline protease production by batch culture of Bacillus,sp.RKY3 through Plackett-Burman and response surface methodological approaches[J].Bioresource Technology,2008,99(7):2242-2249.
[21]	巩宗强,李培军,王新,等.芘在土壤中的共代谢降解研究[J].应用生态学报,2001,12(3):447-450.
[22]	周乐,盛下放.芘降解菌株的筛选及降解条件的研究[J].农业环境科学学报,2006,25(6):1504-1507.
[23]	何晶晶.石油烃污染土壤微生物多样性分析及PAHs降解菌研究[D].乌鲁木齐:新疆农业大学,2008.
[24]	Hayes L A,Nevin K P,Lovley D R.Role of prior exposure on anaerobic degradation of naphthalene and phenanthrene in marine harbor sediments[J].Organic Geochemistry,1999,30(8):937-945.