Shifts of microbial communities during Fe(II) oxidation coupled to nitrate reduction in paddy soil
CHEN Peng-cheng1,2,3, LI Xiao-min2, LI Fang-bai2
1. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;
2. Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China;
3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
A paddy soil which was collected from South China was cultivated in different treatments (Soil, Soil+Fe(Ⅱ), Soil+NO3- and Soil+Fe(Ⅱ)+NO3-) at circumneutral pH under anoxic conditions. The objectives were to investigate the transformations of Fe(Ⅱ) and nitrate as well as the shifts of composition and diversity of microbial communities during these processes. The results revealed that Fe(Ⅱ) could not be oxidized in the treatment of Soil+Fe(Ⅱ), but only be oxidized in the treatment of Soil+Fe(Ⅱ)+NO3-. Meanwhile, the presence of Fe(Ⅱ) slowed down the NO3- reduction. Illumina high throughput sequencing was used to profile the diversity and abundance of microbial communities over time. The results showed that no significant difference of microbial communities between treatments of Soil+Fe(Ⅱ) and Soil. Pseudogulbenkiania, Flavobacterium and Rhodocyclus gradually became the dominant genera in the treatment of Soil+NO3-. Zoogloea, Geothrix, Sunxiuqinia and Vulcanibacillus were the most abundant genera in the treatment of Soil+Fe(Ⅱ)+NO3-, which was a mixture of nitrate reducers, Fe(Ⅱ) oxidizers and Fe(Ⅲ) reducers.
陈鹏程, 李晓敏, 李芳柏. 水稻土Fe(II)氧化耦合NO3-还原的微生物变化[J]. 中国环境科学, 2017, 37(1): 358-366.
CHEN Peng-cheng, LI Xiao-min, LI Fang-bai. Shifts of microbial communities during Fe(II) oxidation coupled to nitrate reduction in paddy soil. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(1): 358-366.
Hauck S,Benz M,Brune A,et al.Ferrous iron oxidation by denitrifying bacteria in profundal sediments of a deep lake (Lake Constance)[J].FEMS Microbiology Ecology,2001,37(2):127-134.
[2]
Kendall B,Anbar A D,Kappler A,et al.The global iron cycle[J].Fundamentals of Geobiology,2012:65-92.
[3]
Lovley D R,Holmes D E,Nevin K.P.Dissimilatory Fe (Ⅲ) and Mn (IV) reduction[J].Advances in microbial physiology,2004,49:219-286.
Ding L J,Su J Q,Xu H J,et al.Long-term nitrogen fertilization of paddy soil shifts iron-reducing microbial community revealed by RNA-(13) C-acetate probing coupled with pyrosequencing[J].Isme Journal,2014,9(3):721-734.
[6]
Posth N R,Canfield D E,Kappler A.Biogenic Fe (Ⅲ) minerals:From formation to diagenesis and preservation in the rock record[J].Earth-Science Reviews,2014,135(4):103-121.
[7]
Kappler A,Straub K L.Geomicrobiological cycling of iron[J].Reviews in Mineralogy and Geochemistry,2005,59(1):85-108.
[8]
Miot J,Benzerara K,Morin G,et al.Iron biomineralization by anaerobic neutrophilic iron-oxidizing bacteria[J].Geochimica et Cosmochimica Acta,2009,73(3):696-711.
[9]
Coby A J,Picardal F,Shelobolina E,et al.Repeated anaerobic microbial redox cycling of iron[J].Applied and Environmental Microbiology,2011,77(17):6036-6042.
[10]
Straub K L,Schönhuber W A,Buchholz-Cleven B E,et al.Diversity of ferrous iron-oxidizing,nitrate-reducing bacteria and their involvement in oxygen-independent iron cycling[J].Geomicrobiology Journal,2004,21(6):371-378.
[11]
Weber K A,Achenbach L A,Coates J D.Microorganisms pumping iron:anaerobic microbial iron oxidation and reduction[J].Nature Reviews Microbiology,2006,4(10):752-764.
[12]
Ratering S,Schnell S.Nitrate-dependent iron (Ⅱ) oxidation in paddy soil[J].Environmental Microbiology,2001,3(2):100-109.
Li X,Zhang W,Liu T,et al.Changes in the composition and diversity of microbial communities during anaerobic nitrate reduction and Fe (Ⅱ) oxidation at circumneutral pH in paddy soil[J].Soil Biology and Biochemistry,2016,94:70-79.
[15]
Rhoderick G C,Dorko W D.Standards development of global warming gas species:Methane,nitrous oxide,trichlorofluoromethane,and dichlorodifluoromethane[J].Environmental Science&Technology,2004,38(9):2685-2692.
[16]
Paul T,Miller P L,Strathmann T J.Visible-light-mediated TiO2 photocatalysis of fluoroquinolone antibacterial agents[J].Environmental Science&Technology,2007,41(13):4720-4727.
[17]
Fadrus H,Malý J.Rapid extraction-photometric determination of traces of iron (Ⅱ) and iron (Ⅲ) in water with 1,10-phenanthroline[J].Analytica Chimica Acta,1975,77:315-316.
Wolfe J P,Wagaw S,Marcoux J F,et al.Nitrogen fixation and dissimilatory nitrate reduction to ammonium (DNRA) support nitrogen dynamics in Texas estuaries.Limnol Oceanogr[J].Limnology&Oceanography,2006,51(1):558-568.
[20]
Mahadevan R,Bond D R,Butler J E,et al.Characterization of metabolism in the Fe (Ⅲ)-reducing organism Geobacter sulfurreducens by constraint-based modeling[J].Applied and Environmental Microbiology,2006,72(2):1558-1568.
[21]
Zhao L,Dong H,Kukkadapu R,et al.Biological oxidation of Fe (Ⅱ) in microbially reduced nontronite coupled with nitrate reduction by Pseudogulbenkiania sp.Strain 2002:Implications for remediation of nitrate contamination in the environment[J].Geochimica et Cosmochimica Acta,2013,119:231-247.
[22]
Horn M A,Ihssen J,Matthies C,et al.Dechloromonas denitrificans sp.nov.,Flavobacterium denitrificans sp.nov.,Paenibacillus anaericanus sp.nov.and Paenibacillus terrae strain MH72,N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa[J].International Journal of Systematic and Evolutionary Microbiology,2005,55(3):1255-1265.
[23]
Reinhold-Hurek B,Hurek T.The genera Azoarcus,Azovibrio,Azospira and Azonexus[M].The prokaryotes.Springer,2006:873-891.
[24]
L'Haridon S,Miroshnichenko M,Kostrikina N,et al.Vulcanibacillus modesticaldus gen.nov.,sp.nov.,a strictly anaerobic,nitrate-reducing bacterium from deep-sea hydrothermal vents[J].International Journal of Systematic and Evolutionary Microbiology,2006,56(5):1047-1053.
[25]
Carlson H K,Clark I C,Blazewicz S J,et al.Fe (Ⅱ) oxidation is an innate capability of nitrate-reducing bacteria that involves abiotic and biotic reactions[J].Journal of Bacteriology,2013,195(14):3260-3268.
[26]
Picardal F.Abiotic and microbial interactions during anaerobic transformations of Fe (Ⅱ) and NOx-[J].Frontiers in Microbiology,2012,3(3):112.
[27]
Nevin K P,Lovley D R.Mechanisms for accessing insoluble Fe (Ⅲ) oxide during dissimilatory Fe (Ⅲ) reduction by Geothrix fermentans[J].Applied and Environmental Microbiology,2002,68(5):2294-2299.
[28]
Kong Y,Nielsen J L,Nielsen P H.Microautoradiographic study of Rhodocyclus-related polyphosphate-accumulating bacteria in full-scale enhanced biological phosphorus removal plants[J].Applied and Environmental Microbiology,2004,70(9):5383-5390.
[29]
Takai K,Abe M,Miyazaki M,et al.Sunxiuqinia faeciviva sp.nov.,a facultatively anaerobic organoheterotroph of the Bacteroidetes isolated from deep subseafloor sediment[J].International Journal of Systematic and Evolutionary Microbiology,2013,63(5):1602-1609.
