Abstract：Organic fraction of municipal solid waste (OFMSW) was pyrolyzed at 700℃ to produce biochar, which was used to amend an organic and nitrogen deficient purple soil in Chongqing. Twelve month pot incubation experiments were conducted to investigate effects of the OFMSW-based biochar on the content of organic matter (OM) and total nitrogen (TN) as well as microbial community structure of the purple soil. The experiments were performed with four treatments of 0%, 1%, 3% and 5% (w/w) of biochar addition, and the microbial communities of the amended soils were analyzed by high throughput sequencing technology. The results showed that the addition of 3% and 5% of biochar significantly increased the content of OM and TN and reduced the alpha diversity of bacteria in purple soils. The principal component analysis showed that the bacterial communities in the 0% and 1% treatments were similar, and the bacterial communities in the 3% and 5% treatments were similar. The Illumina sequencing from 24soil samples identified 42bacteria phyla, 6of which were the main bacterial phyla Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chloroflexi and Gemmatimonadetes, accounting for 83.7%~94.3% of the total number of sequencing. The bacteria phyla varied in their response to the addition levels of biochar. In 24soil samples, 642 genera were identified, among which 105 genera had a relative abundance greater than 1%. The responses of some major bacterial genera to 3% and 5% addition of biochar were different from their responses to 0% and 1% biochar addition.
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ZHANG Shang-yi, LIU Guo-tao, XIE Meng-pei. Influence of organic fraction of municipal solid waste-based biochar on microbial community structure in a purple soil. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(2): 669-676.
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