Dissolved methane dynamics during the degradation of organic matter derived from cyanobacterial bloom
HU Wan-ting1,2, TANG Qian2,3, SUN Wei2,4, ZHU Li-feng1, XING Peng2
1. College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; 2. State Key Laboratory of Lake and Environment, Nanjing Institute of Geography Limnology, Chinese Academy of Sciences, Nanjing 210008, China; 3. University of Chinese Academy of Sciences, Beijing 100039, China; 4. Applied Meteorological Institute, Nanjing University of Information Science and Technology, Nanjing 210044, China
Abstract：In shallow eutrophic lakes, methane production during the decomposition of organic matter derived from cyanobacterial blooms and associated factors for the whole process in the water column remain poorly understood. In this work, in situ mesocosms experiment in Lake Chaohu were carried out to stimulate the Cyanobacteria anaerobic decomposition. During the process, dissolved methane concentration and the main environmental factors were measured at the water bottom. Quantitative real-time PCR method was applied to analyze the abundance of mcrA gene as well as the bacterial and archaeal 16S rRNA genes in each sample. Results showed that with the biomass decomposition, the amount of methanogens increased gradually in accordance with the accumulation of dissolved methane in the water column (maximum concentration 2.94mg/L). A significantly positive correlation was detected between mcrA gene abundance and the dissolved methane concentration. Furthermore, there was also a significantly positive correlation between ferrous ions (Fe2+) concentration and the methanogen mcrA abundance. Our results support for the notion that water column can be a hot spot for the biosynthesis of methane due to the outbreak of cyanobacterial bloom. This study helps to compare the relative contribution of methane production between water column and surface sediments during the cyanobacterial blooms degradation.
Borrel G, Jézéquel D, Biderre-Petit C, et al. Production and consumption of methane in freshwater lake ecosystems[J]. Research in Microbiology, 2011,162(9):832-847.
[3]
Bastviken D, Cole J, Pace M, et al. Methane emissions from lakes:Dependence of lake characteristics, two regional assessments, and a global estimate[J]. Global Biogeochemical Cycles, 2004, 18(4):GB4009.
[4]
Bastviken D, Cole J, Pace M, et al. Fates of methane from different lake habitats:Connecting whole-lake budgets and CH4 emissions[J]. Journal of Geophysical Research Biogeosciences, 2008,113(G2).
[5]
Liikanen A, Martikainen P J. Effect of ammonium and oxygen on methane and nitrous oxide fluxes across sediment-water interface in a eutrophic lake[J]. Chemosphere, 2003,52(8):1287-1293.
Chen Y, Qin B, Teubner K, et al. Long-term dynamics of phytoplankton assemblages:Microcystis-domination in Lake Taihu, a large shallow lake in China[J]. Plankton Research, 2003, 25:445-453.
[9]
Qin B. Lake eutrophication:control countermeasures and recycling exploitation[J]. Ecological Engineering, 2009,35(11):1569-1573.
Falz K Z, Holliger C, Grosskopf R, et al. Vertical distribution of methanogens in the anoxic sediment of Rotsee (Switzerland)[J]. Applied and Environmental Microbiology, 1999,65(6):2402-2408.
[13]
Schwarz J I K, Eckert W, Conrad R. Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel)[J]. Systematic and Applied Microbiology, 2007,30:239-254.
[14]
Kampbell D H, Vandegrift S A. Analysis of dissolved methane, ethane, and ethylene in ground water by a standard gas chromatographic technique[J]. Journal of Chromatographic Science, 1998,36(5):253-256.
[15]
Xing P, Hahn M W, Wu Q L. Low taxon richness of bacterioplankton in high-altitude lakes of the eastern Tibetan Plateau, with a predominance of Bacteroidetes and Synechococcus spp.[J]. Applied and Environmental Microbiology, 2009,75(22):7017-7025.
[16]
Whelan J A, Russell N B, Whelan M A. A method for the absolute quantification of cDNA using real-time PCR[J]. Journal of Immunological Methods, 2003,278(1):261-269.
İnceoğlu Ö, Llirós M, Crowee S A, et al. Vertical distribution of functional Potential and Active microbial communities in meromictic Lake Kivu[J]. Microbial Ecology, 2015,70(3):596-611.
[19]
Luton P E, Wayne J M, Sharp R J, et al. The mcrA gene as an alternative to 16s rRNA in the phylogenetic analysis of methanogen populations in landfill[J]. Microbiology, 2002, 148(11):3521-3530.
[20]
Cavicchioli R. Cold-adapted archaea[J]. Nature Reviews Micro-biology, 2006,4(5):331-343.
[21]
Franzmann P D, Liu Y, Balkwill D L, et al. Methanogenium frigidum sp. nov., a psychrophilic, H2-using methanogen from Ace Lake, Antarctica[J]. International Journal of Systematic and Evolutionary Microbiology, 1997,47(4):1068-1072.
Peng S C, Xue J, Wang J, et al. Iron-enhanced anaerobic digestion of cyanobacterial biomass from Lake Chaohu[J]. Fuel, 2014,117:1-4.
[26]
Boyd E S, Anbar A D, Miller S, et al. A late methanogen origin for molybdenum-dependent nitrogenase[J]. Geobiology, 2011, 9(3):221-232.
[27]
Lacy D, Negash B, Basvapatna S R, et al. Bacterial methanogenesis and growth from CO2 with elemental iron as the sole source of electrons[J]. Science, 1987,237:509-11.
[28]
Zhang J, Dong H, Liu D, et al. Microbial reduction of Fe(III) in illite-smectite minerals by methanogen Methanosarcina mazei[J]. Chemical Geology, 2012,292:35-44.
Bellido J L, Peltomaa E, Ojala A. An urban boreal lake basin as a source of CO2 and CH4[J]. Environmental Pollution, 2011, 159(1):1649-1659.
[33]
Nakamura T, Nojiri Y, Utsumi M, et al. Methane emission to the atmosphere and cycling in a shallow eutrophic lake[J]. Archiv für Hydrobiologie, 1999,144(4):383-407.
[34]
Parkes R J, Webster G, Cragg B A, et al. Deep sub-seafloor prokaryotes stimulated at interfaces over geological time[J]. Nature, 2005,436(7049):390-394.
