The effect of gas phase conditions on short-term preservation effect of AAMMOX bacteria
MA Jin-yuan1, ZUO Lu-shen1, LI Hua-yu1, MA Jin-gui2, XU Ya-ru1, YAO Hong1, YU Hai-qin1, SHAN Dan3
1. School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China;
2. College of Engineering and Technology, Northeast Forestry University, Harbin, 150040, China;
3. Sino-Japanese Friendship Center for Environmental Protection, Beijing 100029, China
The effect of gas phase conditions on the performance of anaerobic ammonium oxidation (ANAMMOX) bacteria under short-term preservation was studied. Under the conditions of normal temperature (14±0.5)℃ and low temperature (4±0.5)℃, ANAMMOX bacteria were preserved in the four gas phase conditions (13393mg/m3 NO, 1339mg/m3 NO, nitrogen and air) for 28days and the recovery experiments of ANAMMOX bacteria preserved under normal temperature condition was studied. The results showed that the activities of the ANAMMOX bacteria preserved in 13393mg/m3 NO and nitrogen conditions were higher than that of the bacteria preserved in the air at the corresponding temperature. The retention rates of ANAMMOX bacteria were 23.40%, 86.22%, 61.54% and 46.47%, respectively,under the four kinds of preservation (normal temperature condition), However, Under low temperature conditions, the retention rates were 36.53%, 47.12%, 53.53% and 42.95%, respectively; The results demonstrated that preservation in oxygen-free environment was beneficial to maintain color and settleability of bacteria while biomass decreased and in high concentrations of NO Can reduce the loss of biomass during preservation. The results of the recovery experiments showed that the distribution of each stage during the recovery process was various in different preservation mode. The adaptation period of bacteria preserved in air was 6cycles while in nitrogen was 2 cycles.
马金元, 左陆珅, 李华宇, 马金贵, 徐雅茹, 姚宏, 于海琴, 山丹. 气相条件对ANAMMOX菌种短期保藏效果的影响[J]. 中国环境科学, 2017, 37(3): 925-931.
MA Jin-yuan, ZUO Lu-shen, LI Hua-yu, MA Jin-gui, XU Ya-ru, YAO Hong, YU Hai-qin, SHAN Dan. The effect of gas phase conditions on short-term preservation effect of AAMMOX bacteria. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(3): 925-931.
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