Measurement of potential nitrification rates in sediments from adjacent waters of Rushan Bay
HE Hui1,2,3, ZHEN Yu2,3,4, MI Tie-zhu2,3,4, YU Zhi-gang3,5
1. College of Marine Life Science, Ocean University of China, Qingdao 266003, China;
2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;
3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
4. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;
5. Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China
In this paper, dissolved inorganic nitrogen (DIN) contents declined significantly when potential nitrification rates (PNR) were measured in sediments from adjacent waters of Rushan Bay, which indicated DIN loss occurred during cultivation, and the ratio of dissolved inorganic nitrogen loss to nitrification content ranged from 2.72% to 40.02%. Moreover, the expressions of copper-containing nitrite reductase gene (nirK) were analyzed with real-time quantitative polymerase chain reaction (qPCR), and the results showed that the gene expressed in both ammmonia-oxidizing archaea (AOA) and aerobic ammonia-oxidizing bacteria (AOB), and nitrifier denitrification was one of the reasons that led to DIN loss. PNR would be underestimated if only nitrate and nitrite concentrations were taken into account. For station C0 and C2, total PNR considered ND was 15.9 and 22.1 times of that not considered ND, respectively; and PNR of AOA considered ND was 22.3 and 46.1 times of that not considered ND, respectively. Therefore, DIN loss should be considered when PNR in sediments from adjacent waters of Rushan Bay were calculated.
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