1. College of Resources and Environment Engineering, Wuhan University of Technology, Wuhan 430070, China;
2. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510640, China;
3. College of Resources and Environment Engineering, Wuhan University of Science and Technology, Wuhan 430081, China;
4. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China;
5. The key Laboratory of Water and Air Pollution Control of Guangdong Province, Guangzhou 510655, China
Sensitivity and specificity have been used to assess the performance of fecal indicator in microbial source tracking. However, it is also important to characterize the stability of fecal indicators in aquatic habitats. In this study, indicators decay microcosm in aerobic circumstance was deployed. Decay characteristics of Escherichia coli(EC), Bacteroides (GB) and Chicken-specific marker(CB) in different nutrition condition was evaluated based on real-time quantitative PCR assay. The results indicated that decay rate of three indicators was fit to first-order kinetics. In the control group and experimental system that contain high nutrients levels indicates the attenuation coefficient k of EC (0.135vs. 0.134, the former as the control group, below the same as above), GB (0.415vs. 0.457), CB (0.425vs. 0.437) respectively. The effect of nutrition level on the decay rate of EC, GB and CB was inconspicuous. EC decayed gradually when compared with the severe attenuation of BC and CB. Although significant mutual linear correlationship of different indicators was observed, different characteristics of each indicator related to decay rate should specially be taken into consideration for further application.
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