Microbial diversity in activated sludges of conventional and reversed A2/O processes
LI Peng1,2, BI Xue-jun3, WANG Jun2, RU Shao-guo2
1. Shandong Academy of Environmental Science, Jinan 250013, China;
2. Marine Life Science College, Ocean University of China, Qingdao 266003, China;
3. School of Environmental and Municipal Engineering, Qingdao Technological University, Qingdao 266033, China
To explore the reason why removal efficiency of nitrogen and phosphorus in reversed anoxic-anaerobic-oxic (A2/O) process is higher than that in conventional A2/O process, bacterial community compositions of activated sludges from the two procedures were investigated by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) technique and cultivation method. Betaproteobacteria and Gammaproteobacteria were detected as dominant bacteria in activated sludge from both processes. In the activated sludge of the reversed A2/O process, a large amount of Nitrosospira sp., Rhodocyclus sp., and four uncultured bacteroidetes bacterium were detected by PCR-DGGE technique, while less of these bacteria were found in the conventional A2/O process. Shewanella algae sp., Klebsiella sp., and Rhodobacter sphaeroides of Gammaproteobacteria were separated from the activated sludge of reversed A2/O process by cultivation method, while they were absent from the conventional A2/O process. The diversity of these above-mentioned bacteria species could account for the higher removal efficiency of nitrogen and phosphorus in the reversed A2/O process. Additionally, scanning electron microscopy showed that the activated sludge of reversed A2/O process was relatively loose, with less Filamentous bacteria and no Nostoc.
李鹏, 毕学军, 王军, 汝少国. 常规和倒置A2/O工艺活性污泥微生物群落结构的比较[J]. 中国环境科学, 2017, 37(3): 1137-1145.
LI Peng, BI Xue-jun, WANG Jun, RU Shao-guo. Microbial diversity in activated sludges of conventional and reversed A2/O processes. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(3): 1137-1145.
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