Nitrogen removal performance of SNAD biofilm cultured by domestic wastewater
ZHENG Zhao-ming, LI Jun, HOU Ai-yue, MA Jing, DU Jia, ZHAO Bai-hang, YANG Jing-yue
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China
The nitrogen removal performance of simultaneous partial nitrification, anaerobic ammonium oxidization and denitrification (SNAD) biofilm was investigated in batch tests. The SNAD biofilm performed good anammox and denitrification activities. The NH4+-N, NO2--N and total inorganic nitrogen (TIN) removal rates of anammox activity were 0.121, 0.180 and 0.267kg N/(kg VSS·d), respectively. The denitrification and nitrite oxidation activities were 0.211 and 0.053kg NO2--N/(kg VSS·d), respectively. The suitable pH ranges for anammox activity were 5.0 to 9.0. The the inhibition effect of pH on the anammox bacteria could be largely relieved by biofilm. Besides, the SNAD biofilm was found to perform high tolerance to the inhibition of NO2--N and free nitrous acid (FNA). The NH4+-N removal rates for anammox process were 0.087 and 0.029kg N/(kg VSS·d) with the NO2--N concentrations of 100mg/L (FNA concentration of 70μg/L) and 150mg/L (FNA concentration of 100μg/L), respectively. The SEM observations indicated that the bacteria in the outer part of the SNAD biofilm were mainly short rod-shaped. In the inner part of the SNAD biofilm, the bacteria were mainly crater-shaped, which should be anammox bacteria.
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