Community structure and influencing factors of bacterioplankton in the Main Cancel of the Mid-line Project of South-to-North Water Division in sections of Henan Province.
CHEN Zhao-jin1,2, CHEN Hai-yan2,3, LI Yu-ying1,2, HUANG Jin2,3, LU Kai-jie4, ZHAO Hai-jun2,3, LI Bing2,3, ZHU Jing-ya1,2, HU Lan-qun2,3
1. Key Laboratory of Ecological Security for Water Source Region of Mid-line Project of South-to-North Diversion Project of Henan Province, College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;
2. Henan Collaborative Innovation Center of Water Security for Water Source Region of Mid-line Project of South-to-North Diversion Project, Nanyang 473061, China;
3. Emergency Centre for Environmental Monitoring of Mid-line Project of South to North Water Division, Xichuan 474475, China;
4. Office of South to North Water Diversion of Fangcheng County, Fangcheng 473200, China
To determine which factors may have an impact on the community composition of bacterioplankton, 15surface water samples were collected from the main cancel of the Mid-line Project of South-to-North Water Division in 5sections of Henan province, in July 2016, and 7essential environmental factors were evaluated. The results showed that water quality in the main cancel was strongly influenced by total nitrogen (TN) and the permanganate index (CODMn). Water system in Qushou, Fangcheng, and Xinzheng sections was under the mesotrophic state, and in Wenxian and Anyang sections, under the light eutrophic state. High-throughput sequencing was used to analyze distribution characteristics of the community structure, diversity of bacterioplankton. Phylogenetic analysis based on 16S ribosomal DNA sequences revealed that bacterioplankton could be subdivided into 25major phylogenetic and 407genus groups. The dominant phylogenetic groups included Actinobacteria, Proteobacteria, Planctomycetes, Bacteroidetes, Cyanobacteria, Verrucomicrobia, and Armatimonadetes. Diversity of the bacterioplankton community in the main cancel of the Mid-line Project of South-to-North Water Division could be ranked in the following order: Wenxian > Xinzheng > Fangcheng > Qushou > Anyang. The results of canonical correspondence analysis (CCA) of the relation between the bacterioplankton and 7 environmental factors showed that TN, ammonia nitrogen (NH4+-N), chlorophyll a (Chl a), chemical oxygen demand (COD), and the CODMn were the main environmental factors affecting the distribution characteristics of the bacterioplankton community.
陈兆进, 陈海燕, 李玉英, 黄进, 鲁开杰, 赵海军, 李冰, 朱静亚, 胡兰群. 南水北调中线干渠（河南段）浮游细菌群落组成及影响因素[J]. 中国环境科学, 2017, 37(4): 1505-1513.
CHEN Zhao-jin, CHEN Hai-yan, LI Yu-ying, HUANG Jin, LU Kai-jie, ZHAO Hai-jun, LI Bing, ZHU Jing-ya, HU Lan-qun. Community structure and influencing factors of bacterioplankton in the Main Cancel of the Mid-line Project of South-to-North Water Division in sections of Henan Province.. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(4): 1505-1513.
Newton R J, Jones S E, Eiler A, et al. A guide to the natural history of freshwater lake bacteria[J]. Microbiology and Molecular Biology Reviews, 2011,75(1):14-49.
van Dijk E L, Auger H, Jaszczyszyn Y, et al. Ten years of next-generation sequencing technology[J]. Trends in Genetics, 2014,30(9):418-426.
[8]
Fan L M, Barry K, Hu G D, et al. Bacterioplankton community analysis in tilapia ponds by Illumina high-throughput sequencing[J]. World Journal of Microbiology and Biotechnology, 2015,32(1):1-11.
Yu L Y, Zhang W J, Liu L M, et al. Determining microeukaryotic plankton community around xiamen island, southeast china, using Illumina MiSeq and PCR-DGGE techniques[J]. PLoS ONE, 2015,10(5):e0127721.
[12]
Liu L M, Yang J, Yu Z, et al. The biogeography of abundant and rare bacterioplankton in the lakes and reservoirs of China[J]. ISME Journal, 2015:2068-2077.
Caporaso J G, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods, 2010,7(5):335-336.
[17]
Schloss P D, Westcott S L, Ryabin T, et al. Introducing mothur:open-source, platform-independent, community-supported software for describing and comparing microbial communities[J]. Applied and Environmental Microbiology, 2009,75(23):7537-7541.
[18]
Segata N, Izard J, Waldron L, et al. Metagenomic biomarker discovery and explanation[J]. Genome Biology, 2011,12(6):1-18.
[19]
Ter Braak C J, Smilauer P. CANOCO reference manual and CanoDraw for Windows user's guide:software for canonical community ordination (version 4.5)[M]. Microcomputer Power, Ithaca, NY, 2002.
Niu Y, Shen H, Chen J, et al. Phytoplankton community succession shaping bacterioplankton community composition in Lake Taihu, China[J]. Water Research, 2011,45(14):4169-4182.
[29]
Dai Y, Yang Y Y, Wu Z, et al. Spatiotemporal variation of planktonic and sediment bacterial assemblages in two plateau freshwater lakes at different trophic status[J]. Applied Microbiology and Biotechnology, 2016,100(9):4161-4175.
Haukka K, Kolmonen E, Hyder R, et al. Effect of nutrient loading on bacterioplankton community composition in lake Mesocosms[J]. Microbial Ecology, 2006,51(2):137-146.
