The characteristic of activated sludge in nitrifying low-DO reactor
GAO Yao-yuan, PENG Yong-zhen, BAO Peng, GUO Si-yu, WANG Shu-ying
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China
The characteristics and microbial community of nitrifying bacteria cultivated at the low DO concentration of 0.3~0.5mg/L in a sequencing batch reactor (SBR) was investigated in this study. The bacterial abundance and diversity were analysed with different influencing factors after successful cultivation of the active sludge. Results showed that, the optimum temperature for low-DO nitrifying bacteria was 25℃, and the optimum pH was 8.5. Under the condition of pH 7.5 at 20℃, specific ammonia oxidate rate and specific nitrite oxidate rate were 0.3 and 0.6mgN/(mgMLSS×d) with DO of 0.5~4mg/L, respectively. High throughput sequencing analysis revealed that Nitrospira genus was the dominant nitrite oxidizing bacteria (NOB) accounting for 33% of whole community, and Nitrosomonas was the major ammonium oxidizing bacteria (AOB) accounting for 7% in the low-DO nitrification reactor.
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