The startup of simultaneous anammox and denitrification process atdifferent operational phases of anammox
LI Dong1, YANG Qiao-yun1, ZENG Hui-ping1, ZHANG Jie1,2
1. Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
In order to start up astable simultaneous anammox and heterotrophic denitrification process to treat domestic sewage, the experiment was carried out at different ammonia nitrogen removal of the load (ANR). During the operation period, the variation of ammonia nitrogen (NH4+-N)nitrite nitrogen (NO2--N) and nitrate nitrogen (NO3--N) and dynamics activities of functional microbes were investigated to elaboratemain factors for the SAD process. The results showed that the reactor could treat domestic wastewater containing 100~150mg/L COD when the ANR was during 0.27~0.40kg/(μ3·d) and it could treat domestic wastewater containing 100~200mg/L COD stably when the ANR was 0.85kg/(m3·d) The reactor could start up stable SAD process if the ratioof ananmmox bacteria dynamics activity (μNH4+-N)with heterotrophicbacteria (μCOD)and denitrificationbacteria (μNH3--N) was within reasonable limits.The anammox bacteria activity was increased ,meanwhile the heterotrophicbacteria and denitrificationbacteriaactivity were decreased when the hydraulic retention time (HRT) was reduced. According to the cycle experiments,the simultaneous anammox and partial denitrification process
李冬, 杨巧云, 曾辉平, 张杰. SAD工艺在厌氧氨氧化不同运行阶段的启动[J]. 中国环境科学, 2017, 37(5): 1775-1782.
LI Dong, YANG Qiao-yun, ZENG Hui-ping, ZHANG Jie. The startup of simultaneous anammox and denitrification process atdifferent operational phases of anammox. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(5): 1775-1782.
Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms [J]. Applied Microbiology & Biotechnology, 1998,50(5):589-596.
[2]
Górka M, Zwolińska E, Malkiewicz M. New anaerobic process of nitrogen removal. [J]. Water Science & Technology A Journal of the International Association on Water Pollution Research, 2006,54(8):163-170.
[3]
Tal Y, Watts J E, Schreier H J. Anaerobic ammonium-oxidizing (anammox) bacteria and associated activity in fixed-film biofilters of a marine recirculating aquaculture system. [J]. Applied & Environmental Microbiology, 2006,72(4):2896-2904.
Pathak B K, Kazama F, Tanaka Y, et al. Quantification of anammox populations enriched in an immobilized microbial consortium with low levels of ammonium nitrogen and at low temperature. [J]. Applied Microbiology & Biotechnology, 2007,76(5):1173-1179.
[7]
Ni S Q, Ni J Y, Hu D L, et al. Effect of organic matter on the performance of granular anammox process [J]. Bioresource Technology, 2012,110(110):701-705.
[8]
Cydzik-Kwiatkowska A, Rusanowska P, Zielińska M, et al. Structure of nitrogen-converting communities induced by hydraulic retention time and COD/N ratio in constantly aerated granular sludge reactors treating digester supernatant [J]. Bioresource Technology, 2014,154(2):162-170.
Van D G A A. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor. Microbiology (UK) [J]. Microbiology, 1996,142(8):2187-2196.
Wyffels S, Boeckx P, Pynaert K, et al. Nitrogen removal from sludge reject water by a two-stage oxygen-limited autotrophic nitrification denitrification process. [J]. Water Science & Technology A Journal of the International Association on Water Pollution Research, 2004,49(5/6):57-64.
Bi Z, Takekawa M, Park G, et al. Effects of the C/N ratio and bacterial populations on nitrogen removal in the simultaneous anammox and heterotrophic denitrification process: Mathematic modeling and batch experiments [J]. Chemical Engineering Journal, 2015,280:606-613.
Matsumoto S, Katoku M, Saeki G, et al. Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses [J]. Environmental Microbiology, 2010,12(1):192-206.
[19]
Sumino T, Isaka K, Ikuta H, et al. Nitrogen removal from wastewater using simultaneous nitrate reduction and anaerobic ammonium oxidation in single reactor [J]. Journal of Bioscience & Bioengineering, 2006,102(4):346-351.
[20]
Rui D, Peng Y, Cao S, et al. Advanced nitrogen removal from wastewater by combining anammox with partial denitrification [J]. Bioresource Technology, 2014,179C(179C):497-504.