High-throughput sequencing analysis of community structure in reactor enhanced by heterotrophic nitrification-aerobic denitrification bacteria XH02
HUANG Zheng-zheng1, CAO Gang1,2, LI Zi-hui1, CHEN Hai-sheng1, MO Ce-hui1,2
1. School of Environment, Jinan University, Guangzhou 510630, China;
2. Guangdong Engineering Center for Environment Contamination Control and Restorative Materials, Guangzhou 510630, China
To improve the denitrification of bio-reactor and reveal dynamic changes of microbial community structure over time, the microbial community structure and diversity were analyzed by high-throughput sequencing technology in different stages of the SBR reactor inoculated with heterotrophic nitrification-aerobic denitrification XH02. Results showed that the removal rates of TN and COD increased by more than 15% and 10%, respectively. The relative abundance and diversity of microbial flora on the genus level decreased at first and then increased. XH02 exerted a great influence on the microbial community structure of indigenous microorganisms, leading to a significant decrease in the relative abundance of Acinetobacter, Blvii28 and Aquabactenium, but a visible increase in the relative abundance of Treponema and Fontibacter. The relative abundance of XH02 increased gradually with the operation of the reactor until a relatively stable flora was finally established. The SBR operation was roughly divided into four stages based on the results of principal component analysis (PCA) and UPGMA cluster analysis.
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