Effect of C8-oxo-HSL on the growth of P. aeruginosa in wastewater treatment
ZHOU Li-jie1,2,3, WANG Xin3, HAN Qian1, ZHU Ting-ting1, CHENG Gong1, XIA Si-qing3
1. State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology, Shenzhen Key Laboratory of Drinking Water Source Safety Control, Shenzhen Key Laboratory of Emerging Contaminants Detection & Control in Water Environment, Shenzhen Academy of Environmental Sciences, Shenzhen 518001, China;
2. College of Chemistry and Emvironmental Engineering, Shenzhen University, Shonzhen 518060, China;
3. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
N-(3-oxo octanoyl)-L-homoserine lactone (C8-oxo-HSL) is a commonly detected quorum sensing molecule in both natural and built environments. Although it is known that Pseudomonas aeruginosa CICC 23618 can secrete C8-oxo-HSL, behavioral changes of P. aeruginosa under the influence of C8-oxo-HSL remain unelucidated. Due to unique bioactivities often harbored by P. aeruginosa species important to many biological processes (e.g., activated sludge process), it is fundamentally important to understand effects of C8-oxo-HSL to P. aeruginosa. The study showed that C8-oxo-HSL at as low as 10-10g/L level could enhance the growth rate of P. aeruginosa. Thus, it is rational to predict that the growth of gram-negative bacteria bearing the LuxI/R quorum sensing system might also be enhanced by HSL molecules at low concentrations. The study also observed that 10-7g/L C8-oxo-HSL was sufficient to cause P. aeruginosa to secrete a relatively high amount of proteins into its tightly bound EPS, but the production of polysaccharide unaffected by C8-oxo-HSL. Biofilm formation is a complex biological process involving combined effects of cell growth and EPS production. Given the effects mentioned above, it is not surprised to see that 10-8g/L C8-oxo-HSL eventually enhanced the formation of P. aeruginosa biofilm and improved the stability of its cell aggregates. Overall, the study quantitatively demonstrated how C8-oxo-HSL as a representative of HSL quorum sensing family impacted the physiology of P. aeruginosa and revealed its potential implications in biological processes.
周礼杰, 王鑫, 韩倩, 朱婷婷, 成功, 夏四清. C8-oxo-HSL对P. aeruginosa在污水处理中生长特性影响[J]. 中国环境科学, 2017, 37(5): 1930-1936.
ZHOU Li-jie, WANG Xin, HAN Qian, ZHU Ting-ting, CHENG Gong, XIA Si-qing. Effect of C8-oxo-HSL on the growth of P. aeruginosa in wastewater treatment. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(5): 1930-1936.
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