Effects of o-aminophenol on sludge yield and microbial activity
WEI Xue-yu1,2, LIU Zhi-gang2,3, YAN Yu-tao2, XU Xiao-ping1, WANG Xiao-ju2
1. The School of Civil Engineering and Architecture, Anhui Polytechnic University, Wuhu 241000, China;
2. College of Environment, Hohai University, Nanjing 210098, China;
3. Ningbo Water Supply Co., Ltd, Ningbo 315041, China
In order to view sludge reduction and estimate the inhibitory effect of chemical uncoupler on the activity of sludge,the influence of o-aminophenol (oAP) addition on efficiency of sludge reduction.The microbial activity and variation of removal efficiency in NH4+-N and CODCr induced by addition oAP were investigated through batch tests.Four indexes,TTC-electron transport system activity (TTC-ETS),INT-electron transport system activity (INT-ETS),ammonia uptake rate (AUR) and specific oxygen uptake rate (SOUR) were used to characterize the inhibitory effects of the metabolic uncoupler on the sludge activity.The results showed that the average apparent sludge yield of Yobs was decreased from 0.443 to 0.256MLSS/mgCOD for 42.20%,when oAP concentration was set as 12mg/L.The inhibitory effect of oAP on removing NH4+-N was more significant than on decomposing organics,and nitrifying bacteria were more sensitive than heterotrophic bacteria to the inhibitory effects of oAP,which showed the toxic influence on the activity of sludge.Through comparing the inhibition rates of NH4+-N removal efficiency,AUR is supposed to be the most effective index to characterize the inhibitory effects of metabolic uncoupler.Besides,TTC-ETS activity can be used as the optimum index to characterize inhibitory effects of oAP,because its median inhibitory concentration was the minimum of 35.51mg/L,as the minimum one among the four indexes.
韦学玉, 刘志刚, 闫玉涛, 徐晓平, 王晓菊. 邻氨基苯酚对污泥产率及微生物活性的影响[J]. 中国环境科学, 2017, 37(7): 2550-2556.
WEI Xue-yu, LIU Zhi-gang, YAN Yu-tao, XU Xiao-ping, WANG Xiao-ju. Effects of o-aminophenol on sludge yield and microbial activity. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(7): 2550-2556.
Guo W Q, Yang S S, Xiang W S, et al. minimization of excess sludge production by in-situ activated sludge treatment processes-A comprehensive review[J]. Biotechnology Advances, 2013,31,1386-1396.
Lin S, Jiang W J, Tang Q, et al. Impact of a metabolic uncoupler2,4-dichlorophenol on minimization of activated sludge production in membrane bioreactor[J]. Water Science and Technology, 2010,62(6):1379-1385.
[5]
叶芬霞,解偶联代谢对活性污泥工艺中剩余污泥的减量化作用[D]. 杭州:浙江大学, 2003.
[6]
Zuriaga-Agustí E, Mendoza-Roca J A, Bes-Piá A, et al. Sludge reduction by uncoupling metabolism:SBR tests with paranitrophenol and a commercial uncoupler[J]. Journal of Environmental Management, 2016,182:406-411.
[7]
Zheng G H, Li M N, Wang L. Feasibility of 2,4,6-trichlorophenol and malonic acid as metabolic uncoupler for sludge reduction in the sequence batch reactor for treating organic wastewater[J]. Applied Biochemistry and Biotechnology, 2009,144(2):101-109.
[8]
Xie, M L. Utilization of 8kinds of metabolic uncouplers to reduce excess sludge production from the activated sludge process. Master Thesis, Beijing Technology Business University, 2002.
[9]
Chen G H, Mo H K, Liu Y. Utilization of a metabolic uncoupler, 3,3',4',5-tetrachlorosalicylanilide (TCS) to reduce sludge growth in activated sludge culture[J]. Water Research, 2002,36:2077-2083.
[10]
Tian Y, Zhang J, Wu D, et al. Distribution variation of a metabolic Uncoupler 2,6-dichlorophenol (2,6-DCP) n long-term sludge culture and their effects on sludge reduction and biological inhibition[J]. Water Research, 2013,47:279-288.
[11]
Feng X C, Guo W Q, Yang S S, et al. Possible causes of excess sludge reduction adding metabolic uncoupler 3,3',4',5-tetrachlorosalicylanilide (TCS) in sequence batch reactors[J]. Bioresource Technology, 2014,173:96-103.
[12]
Wang W, Li X C, Wang P F, et al. Long-term effects of Ni (Ⅱ) on the performance and activity of activated sludge processes[J]. Ecotoxicology Environmental Safety, 2013,92:144-149.
[13]
Stasinakis A S, Mamais D, Thomaidis N S, et al. Inhibitory effect of triclosan and nonylphenol on respirationrates and ammonia removal in activated sludge systems[J]. Ecotoxicology and Environmental Safety, 2008,70(2):199-206.
[14]
Chen G H, Mo H K, Liu Y. Utilization of a metabolic uncoupler3,3',4',5-retrachlorosalicylanilide (TCS) to reduce sludge growth in activated sludge culture[J]. Water Research, 2002,36(8):2077-2083.
[15]
Lowry O H, Rosebrough N J, Farr A L, et al. Protein measurement with the folin phenol reagent[J]. Journal of Biological. Chemistry, 1951,193:265-275.
Low E W, Chase H A. The use of chemical uncouplers for reducing biomass production during biodegradation[J]. Water Science and Technology, 1998,37(45):399-402.
[18]
Low E W, Chase H A, Milner M G, et al. Uncoupling of metabolism to reduce biomass production in the activated sludge process[J]. Water Research, 2000,34(12):3204-32l2.
[19]
Low E W and Chase H A. Reducing production of excess biomass during wastewater treatment[J]. Water Research, 1999, 33:1119-1132.
[20]
Li P, Li H C, Li J, et al. Evaluation of sludge reduction of three metabolic uncouplers in laboratory-scale anaerobic-anoxic-oxic process[J]. Bioresource Technology, 2016,22:131-36.
[21]
Zhang J, Tian Y, Zuo W, et al. Inhibition of nitrification by the metabolic uncoupler, 2, 6-dichlorophenol (2,6-DCP) in a sequencing batch reactor[J]. Chemical Engineering Journal, 2013, 233:132-137.
[22]
Zuriaga-Agustí E, Garrido-Mauri G, Mendoza-Roca J A, et al. Reduction of the sludge production in a sequencing batch reactor by addition of chlorine dioxide:Influence on the process performance[J]. Chemical Engineering Journal, 2012,209:318-324.
[23]
Ren S J. Assessing wastewater toxicity to activated sludge:recent research and developments[J]. Environment International, 2004, 30:1151-1164.
[24]
Radnieckl T S, Stankus D P, Neigh A, et al. Influence of liberated silver from silver nanoparticles on nitrification inhibition of nitrosomonas europaea[J]. Chemosphere, 2011,85:43-49.
[25]
Katipoglu T Y, Pala I O, Ubay E C, et al. Acute impact of erythromycin and tetracycline on the kinetics of nitrification and organic carbon removal in mixed microbial culture[J]. Bioresource Technology, 2013,144:410-419.
[26]
Han J C, Liu Y, Liu X, et al. The effect of continuous Zn() Ⅱ exposure on the organic degradation capability and soluble microbial products (SMP) of activated sludge[J]. Journal of Hazardous Materials, 2013,244/245(2):489-494.
Frolund B, Palmgren R, Keiding K, et al. Extraction of extracellular polymers from activated sludge using a cation exchange resin[J]. Water Research, 1996,30:1749-1758.
[30]
Wang Y P, Yu S S, Li J, et al. Tuning microbial electrogenic activity by uncouplers[J]. Process Biochemistry, 2016,51:1885-1889.
[31]
Feng B, Fang Z, Hou J C, et al. Effects of heavy metal wastewater on the anoxic/aerobic-membrane bioreactor bioprocess and membrane fouling[J]. Bioresource Technology, 2013,142(8):32-38.
[32]
Zhang J, Tian Y, Zuo W, et al. Inhibition of nitrification by the metabolic uncoupler, 2,6-dichlorophenol (2,6-DCP) in a sequencing batch reactor[J]. Chemical Engineering Journal, 2013,233:132-37.
