Nitrite accumulation properties of partial denitrificationwith methanolas carbon source
NIU Meng, WANG Shu-ying, DU Rui, CAO Shen-bin, PENG Yong-zhen
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China
Taking partial denitrification sludge as seeding sludge, which could reduce nitrate (NO3--N) to nitrite (NO2--N) with stable NO2--N accumulation. NO2--N accumulation properties of partial denitrification with different carbon nitrogen ratio (C/N), NO3--N and pH were studied by batch experiments with methanol as carbon source. The results showed that C/N had no significant effect on the maximum accumulation of NO2--Nor specific denitrification rate when C/N was 2.5~5. When C/N was 2.5, NO2--N accumulation rate was 89% and NO2--N was not reduced. But when C/N>3, NO2--N would be reduced after NO2--N accumulated to the maximum value. In the case of C/N was 2.5 and NO3--Nwas 14.96~58.71mg/L, specific denitrification rate increased with the increase of NO3--N. Continue to improve NO3--N, the change of specific denitrification rate was very small. On the other hand, when NO3--N>29.01mg/L, the higher NO3--N was, the lower C/N required. PH had a significant effect on the maximum accumulation of NO2--N and the specific denitrification rate in the partial denitrification process. When pH=7, NO2--Naccumulated rate、specific NO3--N reduction rateand specific NO2--N accumulation rate was 24.49mg/L、18.96 (mg/(gVSS·h))、18.91 (mg/(gVSS·h)). And then followed byp H=6、8、9. When pH=5 and 10, the partial denitrification process could not be carried out. This study could provide a theoretical basis for the realization and application of partial denitrification.
Ge S, Peng Y, Wang S, et al. Nitrite accumulation under constant temperature in aNOxic denitrification process:The effects of carbon sources and COD/NO3--N[J]. Bioresource Technology, 2012,114:137-143.
[2]
Cao S, Wang S, Peng Y, et al. Achieving partial denitrification with sludge fermentation liquid as carbon source:The effect of seeding sludge[J]. Bioresource Technology, 2013,149:570-574.
[3]
Cao S, Li B, Du R, et al. Nitrite production in a partial denitrifying upflow sludge bed (USB) reactor equipped with gas automatic circulation (GAC)[J]. Water Research, 2016,90:309-316.
[4]
Sun H, Yang Q, Peng Y, et al. Nitrite Accumulation during the Denitrification Process in SBR for the Treatment of Pre-treated Landfill Leachate[J]. Chinese Journal of Chemical Engineering, 2009,17(6):1027-1031.
[5]
Vandegraaf A A, Mulder A, Debruijn P, et al. Anaerobic oxidation of ammonium is a biologically mediated process[J]. Applied and Environmental Microbiology, 1995,61(4):1246-1251.
[6]
Cao S, Du R, Niu M, et al. Integrated anaerobic ammonium oxidization with partial denitrification process for advanced nitrogen removal from high-strength wastewater[J]. Bioresource Technology, 2016,221:37-46.
[7]
Cao S, Peng Y, Du R, et al. Feasibility of enhancing the DEnitrifying AMmonium OXidation (DEAMOX) process for nitrogen removal by seeding partial denitrification sludge[J]. Chemosphere, 2016,148:403-407.
[8]
Du R, Cao S, Wang S, et al. Performance of partial denitrification (PD)-ANAMMOX process in simultaneously treating nitrate and low C/N domestic wastewater at low temperature[J]. Bioresource Technology, 2016,219:420-429.
[9]
Du R, Peng Y, Cao S, et al. Advanced nitrogen removal from wastewater by combining anammox with partial denitrification[J]. Bioresource Technology, 2015,179:497-504.
[10]
Sage M, Daufin G, Gesan-Guiziou G. Denitrification potential and rates of complex carbon source from dairy effluents in activated sludge system[J]. Water Research, 2006,40(14):2747-2755.
[11]
Osaka T, Yoshie S, Tsuneda S, et al. Identification of acetate-or methanol-assimilating bacteria under nitrate-reducing conditions by stable-isotope probing[J]. Microbial Ecology, 2006,52(2):253-266.
[12]
Sun H, Yang Q, Peng Y, et al. Nitrite Accumulation during the Denitrification Process in SBR for the Treatment of Pre-treated Landfill Leachate[J]. Chinese Journal of Chemical Engineering, 2009,17(6):1027-1031.
[13]
Krishna Mohan T V, Nancharaiah Y V, Venugopalan V P, et al. Effect of C/N ratio on denitrification of high-strength nitrate wastewater in aNOxic granular sludge sequencing batch reactors[J]. Ecological Engineering, 2016,91:441-448.
[14]
Glass C, Silverstein J. Denitrification kinetics of high nitrate concentration water:pH effect on inhibition and nitrite accumulation[J]. Water Research, 1998,32(3):831-839.
Apha. Standard Methods for the Examination of Water and Wastewater[Z]. Washington, DC, USA:American Public Health Association, 1998.
[17]
Xu Y. Volatile fatty acids carbon source for biological[J]. Journal of Environmental Sciences, 1996,8(3):257-268.
[18]
Hamlin H J, Michaels J T, Beaulaton C M, et al. Comparing denitrification rates and carbon sources in commercial scale upflow denitrification biological filters in aquaculture[J]. Aquacultural Engineering, 2008,38(2):79-92.
Hamlin H J, Michaels J T, Beaulaton C M, et al. Comparing denitrification rates and carbon sources in commercial scale upflow denitrification biological filters in aquaculture[J]. Aquacultural Engineering, 2008,38(2):79-92.
Wang S Y, Gao D W, Peng Y Z, et al. Nitrification-denitrification via nitrite for nitrogen removal from high nitrogen soybean wastewater with on-line fuzzy control[J]. Water Scienceand Technology, 2004,49(5/6):121-127.
[24]
Peng Y Z, Chen Y, Peng C Y, et al. Nitrite accumulation by aeration controlled in sequencing batch reactors treating domestic wastewater[J]. Water Science and Technology, 2004,50(10):35-43.
[25]
Yang Q, Peng Y, Liu X, et al. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities[J]. Environmental Science & Technology, 2007,41(23):8159-8164.
[26]
Peng Y Z, Gao J F, Wang S Y, et al. Use pH and ORP as fuzzy control parameters of denitrification in SBR process.[J]. Water Science & Technology A Journal of the International Association on Water Pollution Research, 2002,46(4/5):131.
[27]
Gao D W, Peng Y Z, Liang H, et al. Using oxidation-reduction potential (ORP) and pH value for process control of shortcut nitrification-denitrification[J]. Journal of Environmental Science and Health Part A-toxic/hazardous Substances& Environmental Engineering, 2003,38(12):2933-2942.
