advanced Fenton oxidation treatment of tetracycline resistance genes in effluent discharged from biological wastewater treatment
ZENG Ping1, LIU Shi-yue2, ZHANG Jun-ke3, SONG Yong-hui1, LIU Rui-xia1, LIU Yang4
1. Department of Urban Water Environmental Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2. School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China;
3. School of Chemical & Environmental Engineering, China University of Mining and Technology(Beijing), Beijing 100083, China;
4. Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, 7-263, Canada, China
Fenton oxidation was used to remove tetracycline resistance genes (tet genes:tetL and tetE) from wastewater. Changes in the relative abundance of tet genes and the bacterial community structure were monitored using quantity polymerase chain reaction (q-PCR) and denaturing gradient gel electrophoresis (DGGE). The highest removal efficiencies for tetracycline resistance genes and 16SrRNA were achieved at the optimal conditions, including the reaction time of 10min, pH of 5, H2O2/Fe2+ molar ratio of 8 and H2O2 concentration of 0.2mol/L. DGGE analysis showed remarkable changes in the bacterial community diversities after Fenton oxidation. However, no significant correlation was observed either between the removal of tet genes and the parameters used in Fenton oxidation, or between the removal of tet genes and the change in the bacterial community structures. These results may suggested that the removal of tet genes may not only attribute to the removal of tetracycline resistance bacteria. Future investigations may be necessary to further understand the removal mechanisms in details.
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