Study on performance of the Feammox biofilm-reactor
WU Yin1,2, CHEN Chen2, MAO Xiao-yun1, PENG Xiao-chun2
1. College of Natural Resource and Environment, South China Agricultural University, Guangzhou 510642, China;
2. South China Institute of Environmental Science, Ministry of Environment Protection, Guangzhou 510535, China
The microbial reaction of ammonium oxidation coupling with iron reduction (Feammox) were discovered recently. However, little information is found to prove the removal efficiency of ammonium from simulate wastewater in the Feammox biofilm reactor. In this study, the effects of different ammonium concentration on Feammox biofilm reactor under closed condition and their denitrification reaction characteristics under continuous feeding condition were investigated. The results showed that the simulate wastewater with ammonium concentration of 75 mg/L reached the maximum ammonium removal, with the removal rate of 41.49% in 15 days. When the reactor was running continuously for 90 days, with inflowing NH4+-N concentration of 75 mg/L and 10 days HRT, the maximum ammonium removal reached 33.78%. Feammox biofilm reaction can be divided two stages. In first stage (10~60 d), ammonium was possibly oxidized to nitrogen, and the denitrification reaction carried out simultaneously. In second stage (60~90 d), the weakening denitrification reaction resulted in the accumulation of NO3--N. The results of 16SrRNA bacterial sequencing showed that the Feammox reaction was drove by Acidimicrobium and Exiguobacterium.
吴胤, 陈琛, 毛小云, 彭晓春. 基于Feammox的生物膜反应器性能研究[J]. 中国环境科学, 2017, 37(9): 3353-3362.
WU Yin, CHEN Chen, MAO Xiao-yun, PENG Xiao-chun. Study on performance of the Feammox biofilm-reactor. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(9): 3353-3362.
Canfield D E, Glazer A N, Falkowski P G. The evolution and future of Earth's nitrogen cycle[J]. Science, 2010,330(6001):192-196.
[2]
Galloway J N, Townsend A R, Erisman J W, et al. Transformation of the Nitrogen Cycle:Recent Trends, Questions, and Potential Solutions[J]. Science, 2008,320(5878):889-892.
[3]
Strous M, Fuerst J A, Kramer E H, et al. Missing lithotroph identified as new planctomycete[J]. Nature, 1999,400(6743):446-449.
[4]
Quan Z X, Rhee S K, Zuo J E, et al. Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor[J]. Environmental Microbiology, 2008,10(11):3130-3139.
[5]
Jin R C, Yang G F, Yu J J, et al. The inhibition of the Anammox process:A review[J]. Chemical Engineering Journal, 2012, 197(29):67-79.
[6]
Daverey A, Chen Y C, Sung S, et al. Effect of zinc on anammox activity and performance of simultaneous partial nitrification, anammox and denitrification (SNAD) process[J]. Bioresource Technology, 2014,165(8):105-110.
[7]
Clément J C, Shrestha J, Ehrenfeld J G, et al. Ammonium oxidation coupled to dissimilatory reduction of iron under anaerobic conditions in wetland soils[J]. Soil Biology & Biochemistry, 2005,37(12):2323-2328.
[8]
Yang W H, Weber K A, Silver W L. Nitrogen loss from soil through anaerobic ammonium oxidation coupled to iron reduction[J]. Nature Geoscience, 2012,5(8):538-541.
[9]
Ding L J, An X L, Li S, et al. Nitrogen loss through anaerobic ammonium oxidation coupled to iron reduction from paddy soils in a chronosequence[J]. Environmental Science & Technology, 2014,48(18):10641-10647.
[10]
Li X, Hou L, Liu M, et al. Evidence of Nitrogen Loss from Anaerobic Ammonium Oxidation Coupled with Ferric Iron Reduction in an Intertidal Wetland[J]. Environmental Science & Technology, 2015,49(19):11560-11568.
[11]
Huang S, Jaffé P R. Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron-reducing conditions[J]. Biogeosciences, 2015,12(3):769-779.
[12]
Huang S, Chen C, Peng X, et al. Environmental factors affecting the presence of Acidimicriobiaceae and ammonium removal iron-reducing conditions in soil environments[J]. Soil Biology & Biochemistr, 2016,98:148-158.
[13]
Graaf A A V D. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142(8):2187-2196.
Viollier E, Inglett P W, Hunter K, et al. The ferrozine method revisited:Fe(Ⅱ)/Fe(Ⅲ) determination in natural waters[J]. Applied Geochemistry, 2000,15(6):785-790.
Chen Y, Cheng J J, Creamer K S. Inhibition of anaerobic digestion process:A review[J]. Bioresource Technology, 2008, 99(10):4044-4064.
[18]
Sawayama S. Possibility of aNOxic ferric ammonium oxidation[J]. Journal of Bioscience and Bioengineering, 2006,101(1):70-72.
[19]
Holmes D E, Chaudhuri S K, Nevin K P, et al. Microarray and genetic analysis of electron transfer to electrodes in Geobacter sulfurreducens[J]. Environmental Microbiology, 2006,8(10):1805-1815.
[20]
Wang F, Wang J, Jian H, et al. Environmental adaptation:genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3[J]. Journal of Biotechnology, 2008,3(4):1937-1949.
[21]
Albertini M, Vallese F, Valentin M D, et al. The proton iron-sulfur cluster environment of the[FeFe]-hydrogenase maturation protein HydF from Thermotoga neapolitana[J]. International Journal of Hydrogen Energy, 2014,39(32):18574-18582.
[22]
Zhou G, Yang X, Hu L, et al. Electron Shuttles Enhance Anaerobic Ammonium Oxidation Coupled to Iron(Ⅲ) Reduction[J]. Environmental Science & Technology, 2016,50(17):92-98.
[23]
Jensen M M, Thamdrup B, Dalsgaard T. Effects of specific inhibitors on anammox and denitrification in marine sediments[J]. Applied & Environmental Microbiology, 2007,73(10):3151-3183.
[24]
Srensen J. Denitrification rates in a marine sediment as measured by the acetylene inhibition technique[J]. Appl Environ Microbiol, 1978,36(1):139-143.
[25]
Canfield D E, Glazer A N, Falkowski P G. The Evolution and Future of Earth's Nitrogen Cycle[J]. Science, 2010,330(6001):192-196.
[26]
Strous M, Pelletier E, Mangenot S, et al. Deciphering the evolution and metabolism of an anammox bacterium from a community genome[J]. Nature, 2006,440(7085):790-794.
[27]
Chen M Y, Tsay S S, Chen K Y, et al. Pseudoxanthomonas taiwanensis sp. nov. a novel thermophilic, N2O-producing species isolated from hot springs[J]. International Journal of Systematic & Evolutionary Microbiology, 2002,52(6):2155-2161.
[28]
Kesserü P, Kiss I, Bihari Z, et al. Biological denitrification in a continuous-flow pilot bioreactor containing immobilized Pseudomonas butanovora cells[J]. Bioresource Technology, 2003, 87(1):75-80.
Yao S, Ni J, Ma T, et al. Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp. HA2[J]. Bioresource Technology, 2013, 139(13):80-86.
[31]
Su J F, Shi J X, Huang T L, et al. Kinetic analysis of simultaneous denitrification and biomineralization of novel Acinetobacter, sp. CN86[J]. Marine Pollution Bulletin, 2016,109(1):87-94.
[32]
Su J F, Sheng C Z, Huang T L, et al. Characterization of the anaerobic denitrification bacterium Acinetobacter, sp. SZ28and its application for groundwater treatment[J]. Bioresource Technology, 2015,192:654-659.
Wilfert P, Kumar P S, Korving L, et al. The Relevance of Phosphorus and Iron Chemistry to the Recovery of Phosphorus from Wastewater:A Review[J]. Environmental Science & Technology, 2015,49(16):9400-9414.
