Isolation of an electrogen Klebsiella oxytoca d7 and its electricity-generating mechanism
LIU Yue, YUAN Lin-jiang
Key Laboratory of Environmental Engineering, Shaanxi Province, Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an, 710055, China
An electrogen strain Klebsiella oxytoca-d7 was isolated from biofilm on the anode of a stable operating double chamber microbial fuel cell (MFC) using anaerobic double-plate method and its electricity-generating mechanism was investigated in this study. The results show that the bacteria Klebsiella oxytoca d7 performed high electrochemical activity according to the cyclic voltammetry analysis. It carried out extracellular electricigenic respiration in the environment with extracellular electron acceptor. The strain accomplished electricity generating respiration mainly through humus secreted as extracellular electron media detected by the three simensional fluorescence analysis of the strain's metabolite. Ferric reducing experiments implied that excessive ferric ion did not result in extra growth of the strain, and the ferric ion reduction rate of the strain was limited. This research suggests that extracellular respiration is not the mainly respiration path way of the Klebsiella oxytoca d7 under anaerobic condition, but a subsidiary one as of the intracellular respiration in the presence of extracellular electron acceptor in the environment. Even there are enough extracellular electron acceptor in the environment, the strain's growth won't increase significantly. It is considered the strain does not obtain main growth energy from extracellular reduction, but from intracellular respiration. Only a small part of the electrons produced by oxidation of organic is used to reduce the extracellular electron acceptor.
刘玥, 袁林江. 一株产电菌Klebsiella oxytoca d7的分离及产电机理[J]. 中国环境科学, 2017, 37(9): 3540-3548.
LIU Yue, YUAN Lin-jiang. Isolation of an electrogen Klebsiella oxytoca d7 and its electricity-generating mechanism. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(9): 3540-3548.
Chaudhuri S K, Lovley D R. Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells[J]. Nat Biotechnol, 2003,21:1229-1232.
[2]
Logan B E. Exoelectrogenic bacteria that power microbial fuel cells[J]. Nature Reviews Microbiology, 2009,7:375-381.
Lovley D R. Bug Juice. Harvesting electricity with microorganisms[J]. Nat Rev Microbiol, 2006,4:497-508.
[5]
Lovley D R, Nevin K P. Electricity Production with Electricigens[J]. Bioenergy, 2008:295-306.
[6]
Jeffrey A, Gralnick, Dianne K.Newman. Extracellular respiration. Molecular Microbiology, 2007,65(1):1-11.
[7]
Biffinger J C, Byrd J N, Dudley B L et al. Oxygen exposure promotes fuel diversity for Shewanella oneidensis microbial fuel cells[J]. Biosensors & Bioelectronics, 2008,23(6):820-826.
[8]
Newton G J, Mori S, Nakamura R, et al. Analyses of Current-Generating Mechanisms of Shewanella loihica PV-4and Shewanella oneidensis MR-1in Microbial Fuel Cells[J]. Applied & Environmental Microbiology, 2009,75(24):7674-7681.
[9]
Commault A S, Lear G, Packer M A, et al. Influence of anode potentials on selection of Geobacter strains in microbial electrolysis cells[J]. Bioresource Technology, 2013,139C(7):226-234.
[10]
Wang Biao, Huang Jiexun, Zhang Xiaobo, et al. Isolation and Characterization of an Electricity-producing Strain Shewanella sp. S2from Marine[J]. Microbiology China, 2010, 37(3):342-348.
Commault A S, Lear G, Packer M A, et al. Influence of anode potentials on selection of Geobacter strains in microbial electrolysis cells[J]. Bioresource Technology, 2013,139C(7):226-234.
Carmona-Martinez A A, Harnisch F, Fitzgerald L A, et al. Cyclic voltammetric analysis of the electron transfer of Shewanella oneidensis MR-1and nanofilament and cytochrome knock-out mutants[J]. Bioelectrochemistry, 2011,81(2):74-80.
[16]
Paipa C, Mateo M, Godoy I, et al. Comparative study of alternative methods for the simultaneous determination of Fe +3 and Fe+2 in leaching solutions and in acid mine drainages[J]. Minerals Engineering, 2005,18(11):1116-1119.
[17]
Kim C, Ainala S K, Oh Y K, et al. Metabolic flux change in Klebsiella pneumoniae L17 by anaerobic respiration in microbial fuel cell[J]. Biotechnology and Bioprocess Engineering, 2016, 21(2):250-260.
[18]
Deng L F, Li F B, Zhou S G, et al. A study of electron-shuttle mechanism in Klebsiella pneumoniae based-microbial fuel cells[J]. Science Bulletin, 2010,55(1):99-104.
[19]
Kim G T, Webster G, Wimpenny J W, et al. Bacterial community structure, compartmentalization and activity in a microbial fuel cell[J]. Journal of Applied Microbiology, 2006,101(3):698-710.
[20]
Zhang L X, Zhou S G, Zhuang L, et al. Microbial fuel cell based on Klebsiella pneumoniae biofilm. Electrochemistry Communications, 2008,10(10):1641-1643.
[21]
Peng Y, Zhu N, Nie H. Isolation of an electrogen Klebsiella sp. Z6from anodic biofilm and its electricity-generating characteristics[J]. Acta Scientiae Circumstantiae, 2013,33(4):1035-1042.
[22]
Xia X, Cao X X, Liang P. Electricity generation from glucose by a Klebsiella sp. in microbial fuel cells[J]. Applied Microbiology Biotechnology, 2010,87(1):383-390.
[23]
Deng L F, Li F B, Zhou S G, et al. A study of electron-shuttle mechanism in Klebsiella pneumoniae based-microbial fuel cells[J]. Chinese Sci Bull, 2009,54(19):2983-2987.
[24]
Qiao Y, Li C M, Bao S J, et al. Direct electrochemistry and electrocatalytic mechanism of evolved Eschericha coli cells in microbial fuel cells[J]. Chem Commun, 2008,21(11):1290-1292.
[25]
H Zhao, J Zhao. Influence of different operation modes on ammonia nitrogen wastewater treatment of microbial fuel cell[J]. Chemical Industry & Engineering Progress, 2016,35(5):1549-1554.
[26]
X M Li, S G Zhou, F B Li, et al. Fe(Ⅲ) oxide reduction and carbon tetrachloride dechlorination by a newly isolated Klebsiella pneumoniae strain L17[J]. Journal of Applied Microbiology, 2009,106(1):130-139.
[27]
Wu Chun-Yuan, Li Fang-Bai, Zhou S G. Humus respiration and its ecological significance[J]. Acta Ecologica Sinica, 2009, 29(3):1535-1542.
[28]
Yi W, Wang B, Qu D. Diversity of isolates performing Fe(Ⅲ) reduction from paddy soil fed by different organic carbon sources[J]. African Journal of Biotechnology, 2012,(19):4407-4417