Studies on hydrogen production via formate dehydrogenase from Bacillus cereus strains
WANG Hai-Yan1, HAO Rui-Xia2, ZHAO Ya-Qi1, LIU Wei3, CHENG Shui-Yuan1, WANG Mian-Chao1, XU LAN-Ting1
1. Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing 100124, China;
2. College of Construction Engineering, Beijing University of Technology, Beijing 100124, China;
3. College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Hydrogen is a promising clean energy resource. However, the biohydrogen production efficiency needs to be significantly improved to make it competitive to fossil fuels. Formate dehydrogenase, which is a catalyst in the production of 2H+, 2e-, and CO2 from formate, is a critical enzyme in hydrogen production by bacteria. In this study the formate dehydrogenase (fdhF) gene from Bacillus cereus strain XN12 was cloned. The sequencing analysis revealed that the cloned fdhF gene contained 2937base pairs, 39.3% GC content and shared 100% identity with the fdhF gene of Bacillus cereus strain Q1 (genebank No. CP000227.1). To characterize the fdhF gene product of Bacillus cereus strain XN12, the fdhF gene was then subcloned into pET32a and the resulting pET32-FDHF-His plasmid was transformed into Escherichia coli BL21 cells. Through the IPTG induction, the cloned fdhF gene was efficiently overexpressed. The recombinant FdhF protein was highly functional as demonstrated by BV reduction experiment. It was found that the hydrogen production rate of recombinant FdhF protein was greatly influenced by the presence of various metal ions, among which MoO42- and SeO32-increased the hydrogen production mainly by increase recombinant protein expression. The hydrogen production was also higher when glucose used as the substrate than formate used as the substrate. The results suggested that recombinant Bacillus cereus formate dehydrogenase protein was a promising solution for improving biohydrogen production.
Gabrielyan L, Sargsyan H, Trchounian A. Biohydrogen production by purple non-sulfur bacteria Rhodobacter sphaeroides:Effect of low-intensity electromagnetic irradiation[J]. Journal of Photochemistry and Photobiology B, 2016,162(C):592-596.
[2]
Zhang S C, Lai Q H, Lu Y, et al. Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria[J]. Journal of Bioscience and Bioengineering, 2016,122(4):482-487.
[3]
Dhar B R, Elbeshbishy E, Hafez H, et al. Hydrogen production from sugar beet juice using an integrated biohydrogen process of dark fermentation and microbial electrolysis cell[J]. Bioresource Technology, 2015,198:223-230.
Wagner R, Andreesen J R. Differentiation between Clostridium acidiurici and Clostridium cylindrosporum on the basis of specific metal requirements for formate dehydrogenase formation[J]. Archives of Microbiology, 1977,114(3):219-224.
[6]
Maeda T, Sanchez-Torres V, Wood T K. Metabolic engineering to enhance bacterial hydrogen production[J]. Microbial Biotechnology, 2008,1(1):30-39.
Lester R L, Demoss J A. Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli[J]. Journal of Bacteriology, 1971,105(3):1006-1014.
[11]
Enoch H G, Lester R L. Effects of molybdate, tungstate, and selenium compounds on formate dehydrogenase and other enzyme systems in Escherichia coli[J]. Journal of Bacteriology, 1972,110(3):1032-1040.
[12]
Leonhardt U, Andreesen J R. Some properties of formate dehydrogenase, accumulation and incorporation of 185W-tungsten into proteins of Clostridium formicoaceticum[J]. Archives of Microbiology, 1977,115(3):277-284.
[13]
Pinske C, Sawers R G. The importance of iron in the biosynthesis and assembly of[NiFe]-hydrogenases[J]. Biomolecular Concepts, 2014,5(1):55-70.
[14]
Shimizu K. Metabolic regulation of a bacterial cell system with emphasis on Escherichia coli metabolism[J]. ISRN Biochemistry, 2013,2013(6):645983.
[15]
Lara A R, Taymaz-Nikerel H, Mashego M R, et al. Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses[J]. Biotechnology and Bioengineering, 2009,104(6):1153-1161.
[16]
Asano M, Basieva I, Khrennikov A, et al. Quantum-like model for the adaptive dynamics of the genetic regulation of E. coli's metabolism of glucose/lactose[J]. Systems and Synthetic Biology, 2012,6(1/2):1-7.
[17]
Marx G, Miskolci F. The CO2 greenhouse effect and the thermal history of the atmosphere[J]. Advances in Space Research, 1981,1(14):5-18.
[18]
Knox S H, Sturtevant C, Matthes J H, et al. Agricultural peatland restoration:effects of land-use change on greenhouse gas (CO2 and CH4) fluxes in the Sacramento-San Joaquin Delta[J]. Global Change Biology, 2015,21(2):750-765.
[19]
Mandal B, Nath K, Das D. Improvement of biohydrogen production under decreased partial pressure of H2 by Enterobacter cloacae[J]. Biotechnology Letters, 2006,28(11):831-835.
[20]
Li X, Wang Y, Zhang S, et al. Effects of light/dark cycle, mixing pattern and partial pressure of H2 on biohydrogen production by Rhodobacter sphaeroides ZX-5[J]. Bioresource Technology, 2011,102(2):1142-1148.
[21]
Kraemer J T, Bagley D M. Improving the yield from fermentative hydrogen production[J]. Biotechnology Letters, 2007,29(5):685-695.
