Effect of low temperature on nitrogen removal in a sequencing batch biofilm reactor with CANON process
WANG Zhen1, ZHU Zhen-hua2, DING Ya-nan1, WU Shao-xian1, LI Su-qing1, LIU Xiao-xia3
1. Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China;
2. Dezhou Vocational and Technical College, Dezhou 253034, China;
3. Zhejiang Agricultural Technology Extension Center, Hangzhou 310020, China
The treatment performance and underlying molecular mechanisms of nitrogen transformation in a sequencing batch biofilm reactor (SBBR) with the complete autotrophic nitrogen removal over nitrite (CANON) process were investigated at four different low temperatures in this study. After each system achieved the stable stutus in the low temperature environment, different low temperatures resulted in different degrees of variations in the nitrogen removal performance and transformation pathways of the SBBRs, which was mainly because the original dominant bacterial communities for nitrogen removal in the system changed with the temperature during the operation. When the temperature was above 15℃, the abundances and activities of aerobic ammonia oxidizing bacteria (AOB) and anammox bacteria in the SBBR had not been significantly inhibited, the CANON process was the dominant methanism in the nitrogen removal, and the ideal average removal rates of total nitrogen were also achieved. When the temperature was below 15℃, the abundance and activitity of anammox bacteria experienced different degrees of reduction at 10 and 5℃, which led to the change of main pathway for nitrogen transformation in SBBRs, thus the various degrees of deterioration in the nitrogen removal performance. At 10℃, the proliferation and increased activities of nitrite oxidizing bacteria (NOB) made the nitrification/denitrification process replaced CANON to become the primary route of TN removal in the SBBR with the TN removal efficiency of the system declined to (16.87±4.79)%. At 5℃, the stagnation of reduction process in the 1st stage of denitrification and the increased capacity of denitrifiers to compete for NO2--N caused the removal of nitrogen in SBBR to rely on both the CANON process and the nitrification/denitrification process. The nitrogen removal rate at this status was (54.83±3.68)% in the system.
王振, 朱振华, 丁亚男, 吴少贤, 李苏青, 刘晓霞. 低温对CANON型序批式生物膜反应器脱氮的影响[J]. 中国环境科学, 2019, 39(4): 1533-1541.
WANG Zhen, ZHU Zhen-hua, DING Ya-nan, WU Shao-xian, LI Su-qing, LIU Xiao-xia. Effect of low temperature on nitrogen removal in a sequencing batch biofilm reactor with CANON process. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(4): 1533-1541.
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