Isolation of a high-efficient diesel degrading bacteria and its degradation of alkane components of diesel
CHEN Hong-chu, ZHANG Ting-di, FU Yu-feng, RU Jin-tao, QIN Chuan-yu
Key Laboratory of Groundwater Resources and Environment, Ministry of Education, National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, Key Laboratory of Water Resources and Environment, Jilin University, Changchun 130021, China
Abstract：A diesel degrading bacterial strain 13-3isolated from oil-contaminated soil in an oilfield mining area in Northeast China was identified as Acinetobacter sp. by morphological observation and 16S rRNA. The strain could degrade 88.50% diesel of 1g/L cultured at 20℃ at 180r/min for 7days, which showed excellent diesel degrading ability compared with other strains, and the degradation rate of diesel fuel could reach 71.7% after being inoculated into 10g/kg diesel contaminated soil and remediation at 20℃ and soil moisture content of 30% for 21days, which indicated it had great potential in practical diesel-contaminated soil remediation applications. According to the analysis of GC-MS spectrum and the degrading experiments of different chain length alkane substrates, strain 13-3had a wide alkane utilization spectrum (C14~C22), and the degradation rate of C17~C20 could reach more than 90%. The results of environmental factors showed that strain 13-3could grow in the range of pH=6~9, temperature 10~30℃, salinity (NaCl) 0.5%~3%, and still had a high concentration of bacterial solution at the initial diesel concentration of 10g/L, which indicated that strain 13-3 had good environmental adaptability and was expected to exert its degradation performance under complex environmental conditions. Through whole-genome sequencing analysis, three key genes (alkB, almA, ladA) were found in the genome of strain 13-3, and the possible degradation pathway of strain 13-3 was speculated to be the terminal oxidation pathway.
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